Korazbekova K.U. 1,2, Utemisova G.S.1, Zhylysbayeva A.N.1,2
1Regional social and innovative university, Shymkent, Kazakhstan
2M.Auezov South Kazakhstan State University, Shymkent, Kazakhstan
Study of antibacterial properties of
propolis from South Kazakhstan region
Propolis is beekeeping additional minor product. Propolis is used for
therapeutic purposes by people and bees since ancient times. Propolis,
especially in ancient Egypt was a very well-known [1-3]. Propolis is a resin
material with green, brown or green-brown color, and it has positive smell of poplar
bud and smell of honey, vanilla, and honeycombs. Bees collect propolis from bud
of a number of trees and grasses and it is used for slot disinfection. It
contains 55% of the resin, 30% of the wax substances, 10% of the essential oil
and 5% of the pollen rich in vitamins and microelements. Propolis is not yet
fully defined substance, that’s why it has not chemical formula. On the basis
of scientific research and practice known to many years, doctors,
bacteriologists, biologists defined a lot of self-healing qualities of propolis
as a natural product [1-2; 4-5]. In this paper is given research results of
bactericidal properties of propolis taken from South Kazakhstan region.
Research of bactericidal properties of propolis was carried out at the "Bacteriological
Laboratory" of the Clinic of the International Kazakh-Turkish University. Four
types of bacteria such as staphylococcus, diphtheria and dysentery bacillus and
Salmonella typhimurium were used in our experiments. Antibacterial properties
of propolis were studied in 1:1, 1:5, and 1:10 ratio solutions. All cultured
bacteria in Petri dishes were incubated in thermostat and they were observed every
30 minutes, 3 hours, and 24 hours to track changes.
The results of the experiment of the bactericidal properties of propolis
are shown in Table 1.
Propolis solution in the ratio 1: 1 does not show bacterial growth after
30 minutes, 3 hours, and 24 hours of observation in all Petri dishes. Propolis
solution in 1:5 ratio during time points of 30 minutes, 3 hours, and 24 hours showed
positive impact on staphylococcus and diphtheria, but bacterial growth was
revealed in all Petri dishes with cultivation of dysentery bacillus and Salmonella
typhimurium. Observation of antibacterial property of the propolis solution in
1:5 ratios shows positive impact only for diphtheria after 30 minutes, 3 hours,
and 24 hours, but other three bacteria - staphylococcus, dysentery bacillus and
Salmonella continue grow during one day study.
Table 1. Results of the study of antibacterial properties of propolis
|
Name
of the bacteria |
after
30 minutes |
after 3 hours |
after 24 hours |
|||||||||
|
Control culture |
1:1 |
1:5 |
1:10 |
Control culture |
1:1 |
1:5 |
1:10 |
Control culture |
1:1 |
1:5 |
1:10 |
|
|
1. Staphylococcus |
+ |
- |
- |
+ |
+ |
- |
- |
+ |
+ |
- |
- |
+ |
|
2. Diphtheria |
+ |
- |
- |
- |
+ |
- |
- |
- |
+ |
- |
- |
- |
|
3. Dysentery |
+ |
- |
+ |
+ |
+ |
- |
+ |
+ |
+ |
- |
+ |
+ |
|
4. Salmonella typhi murium |
+ |
- |
+ |
+ |
+ |
- |
+ |
+ |
+ |
- |
+ |
+ |
“+” – growth is observed “-” – growth is not
observed
After 24-day observation Petri dishes with cultivated dysentery and
Salmonella in 1:5 and 1:10 ratios solution of propolis were put to the
thermostat for further study. These two bacteria in propolis solution of 1:5
and 1:10 ratio stop their growth only after eight days.
In general, propolis solutions in 1:1 ratio has a powerful effect on
staphylococcus, diphtheria, dysentery, and Salmonella typhimurium within 30
minutes in the experiments carried out to determine the antibacterial
properties of propolis. Propolis solution in 1:5 ratio did not affect only Salmonella
typhimurium and dysentery, ie, growth was not stopped. And propolis solution in
1:10 ratio has affected only to diphtheria. But after 8 days of observation all
three spices of bacteria died in the propolis solution of 1:5 and 1:10 ratio.
The results of experiments show that propolis is a natural product with a
high-quality bactericidal properties.
References
1. Пономарева Е.Г.
Кормовая база пчеловодства и опыление сельскохозяйственных растений. – М.:
Колос, 1980 г . – 255 с.
2. Пономарева Е.Т.,
Детерлеева Н.Б. Медоносные рессурсы и опыление сельскохозяйственных
растений.-М.: Агропромиздат, 1986 г. – 224 с.
3. Прополис: ценный
продукт пчеловодства. – Бухарест: Апимондия, 1987 г.
4. Под ред. Виноградов
Т.В., Зайцев Г.П. Пчела и здоровье человека. –М.: Россельхозиздат, 1964г. – 300
с.
5. Апимондия.
Апитерапия сегодня. Бухарест, 1988г. – 150 с.