Medicine/8.Morphology

Ph.D Shnayder S.A.

Odessa State Medical University, Odessa, Ukraine

Experimental treatment of periodontitis with cryoconservated chordal human blood serum preparation efficacy

Despite numerous fundamental clinical studies the problem of chronic generalized periodontitis is still to be solved. Drugs of various classes are being used to treat chronic generalized periodontitis: anti-inflammation drugs, ant-oxidative drugs, antiseptics, antibiotics, and various combinations of physiotherapeutic treatments. None of the existing treatments provides the complete control of the problem. Novel conservative treatments of periodontitis are being researched. Directed tissue regeneration, growth factors treatment and morphogens application are the methods under development. Nanotechnologies as a part of periodontitis treatment are being researched too. Cell and tissue – based treatments may be beneficial. These treatments are based on the activation of damaged tissues and cells compensatory resources, stimulation of restoration and regeneration mechanisms, replacement of organism’s (organ’s, tissue’s) damaged structures and functions. Natural balanced biologically active compounds are administered. They stimulate various metabolic activities. However, the data concerning cell and tissue treatment of periodontitis are limited.

Study objective: evaluation of cryoconservated chordal human blood serum preparation protective effect on periodontal tissue in animal model.

The Wistar adult male rats were used in the animal model. Two similar groups were used: 1) Decreased mastication periodontitis model, 2) periodontitis model, animals receiving treatment with cryoconservated chordal human blood serum preparation since Day 1. The animals were withdrawn at Days 7, 14, 21 and 30. Endpoints: macro- and microscopic changes of periodontal tissue, dynamic mobility of teeth, mandible alveolar process bone tissue mobility; linear shape of mandible molar roots exposed; relative size of molar roots exposed. Endpoints for mandible gingival mucosa homogenous supernatants: free and protein-binded oxiprolin level, protein-binded/free oxiprolin ratio.

Results: cryoconservated chordal human blood serum preparation inhibits the periodontitis progression. Drug administration prevented bone tissue minerals loss at all stages of the experiment in animals. The relative length of exposed molar root and teeth dynamic mobility at Day 30 were 27.8 and 17.8% lower compared to untreated animals. Histological analysis: in treated animals the gingival and periodontal area mucosa fibers architectonics was less damaged, and gingival mucosa epithelium dyscomplexation was less manifested. Drug administration was associated with the decrease of collagen catabolism intensity level. It was supported by the 9% increase of protein-binded/free oxiprolin ratio in treated animals. Treated animals demonstrated slow rate of epithelial binding area width decrease; gingival mucosa edema, hyperemia and bleeding rate were less manifested and developed later compared to control group.

Cryoconservated chordal human blood serum preparation contains biologically active compounds – hemopoetins, adapogens, opioid peptides, enzymes and reproductive immune system modulators. Indications: chronic inflammation, reparation process disorders (enhanced healing promotion), asthenia, syndrome of chronic fatigue. Periodontitis onset and progression are associated both with local factors of oral cavity and general factor, including disorders of non-specific resistance level. Therefore, the hypothesis is that the study drug could be effective in treatment of periodontitis. The experiment demonstrated that cryoconservated chordal human blood serum provides the effective protection of parodont due to its anti-hypoxic and anti-oxidative properties and the enhancement of reparation processes.

Conclusions. Cryoconservated chordal human blood serum possesses parodonto-protective properties. The drug inhibits the progression of periodontitis clinical manifestations, decreases the rate of inflammation and dystrophic changes of periodontal tissue, inhibits the progression of alveolar process bone tissue mineral loss and decreases the rate of gingival mucosa connective tissue collagen catabolism.