S. K. Severinova, O. M. Lavrovskaya, K. G. Kushnir, L.P. Kolbasina

Crimea state medical university named after S. I. Georgievsky, Simferopol.

State of common lipids in membranes and in blood plasma of rats at experimental models of hypoestrogenemia during complex substituting therapy

Investigation of state of common lipids in erythrocyte membranes and in blood plasma of rats after ovarioectomy and corresponding complex was carried out. During experiment we determinated , that the practical full restorations of cells membranes is in 6 weeks, but in usual conditions biochemical dysbalance of lipids is during all time os experiment.

Introduction

One of topical issues of modern gynecology is the fact of growth quantity gynecologic diseases which demand surgery, especially against rejuvenation of the contigent patients. It is established that isn't dependent on operation volume, at women symptoms of surgical menopause develop in 80% of cases.

Due to the deficiency of an estrogen develops metabolic changes, for example, violations of carbohydrate and lipidic metabolism, a metabolism of bone tissue, deterioration of rheological properties of blood and regulation of vascular tone, psychological frustration. Pathogenic method of treatment is replaceable hormonal therapy which is the only way which stops complications of averaga and heavy degree. One of known method diagnostic efficiency is studing of a condition common lipids in membranes of a erythrocytes and blood. Therefore a research objective is styding of a condition common lipids at rats after an ovarioectomia against complex substituting therapy.

Materials and methods.

Research conducted on 50 females of white rates line “Vistar” who divided into 3 groups. In first group included rates who after a laparotomy with a bilateral ovarioectomy are received complex substuting therapy in form of the preparates “Estrlane”, “Krinon”, “Erbinole”. The second group included rats whom after operation didn't intend replaceable therapy. It was divided by that principle, as the first group. The third group-healthy rats.

Equipment of operations

Operation was performed under a radio anesthesia. After a median laparotomy , a ovary brought out of an abdominal cavity and carried out a bilateral ovarioectomy. After there, abdominal wall powered with an antibiotic and layer-by-layer sewed up.The conditions of process oxidation lipids is estimated on level of common lipids in membranes erythrocytes on V.S. Kamishova's method. Condition of the common lipids in membranes erythrocytes determined by a spektrofotometrical method. The half-scientific material was subject to mathematical processing with criterion removal Student's

Indicators of structures common lipids in membranes of erythrocytes and blood plasma rats after ovarioectomy when using complex substituting therapy.

Indicators	Supervision terms
	2 weeks	4 weeks	6 weeks
Membranes	0,58+(-)0,06 P1<0,01 P2>0,05	0,64+(-)0,09 P1<0,05 P2<0,05	0,73+(-)0,07 P1>0,05 P2<0,01
Plasma	3,38+(-)0,19 P1<0,01 P2>0,05	3,16+(-)0,20 P1<0,05 P2>0,05	2,95+(-)0,18 P1>0,05 P2<0,05

Control:healthy rats, indicators of membrane (0,82+(-) 0,07);plasma (2,64+(-) 0,17).P1 probabiliti P2 intergroup deferences

Indicators of structures common lipids in membranes of erythrocytes and blood plasma of rats after ovarioectomy under usual conditions of content.

Indicators	Supervision terms
	2 weeks	4 weeks	6 weeks
Membranes	0,57+(-)0,08 P1<0,01	0,52+(-)0,06 P1<0,001	0,51+(-)0,09 P1<0,001
Plasma	3,39+(-)0,15 P1<0,01	3,46+(-)0,18 P1<0,01	3,52+(-)0,21 P1<0,001

Control:healthy rats, indicators of membrane (0,82+(-) 0,07);plasma (2,64+(-) 0,17).P1 probabiliti P2 intergroup deferences

Results of research and their discussion.

In 2 weeks after an ovarioectomy of rats as main and control group had an imbalance of lipids. In membranes there were less them 29%-30%, and in plasma increased by 28%. Till 4 weeks in main group stabilazation of biochemical indicatory of blood, where they made on membranes (0,69+(-) 0,2) and in plasma (3,16+(-) 0,20) was observed, in comparison with indicators of condition common lipids in the main group gave doubtfull chracter. Thus, the conducted biochemical researches, allowed to come a conclusion that surgery leads are violation of integrity membranes and washing away lipids. Carrying out complex stabilizes indicate the 4 week, and approaches them to indicators of healthy rats on the 6^th week.

Conclusion

1.Ovariectomy is accompanied by violation of integrity membranes of cells, that leads to decrease in quantity of the common lipids

in membranes of erythrocytes and their grouth in blood plasma.

2. Use of complex substituting therapy has membranestimulation effect.