Ulanova V.A.,
Shchokina K.G.
Department of
Pharmacology and medical toxicology,
National University
of Pharmacy, Kharkiv, Ukraine
The influence of
the extract Humulus lupulus suppositories on the pathogenesis of experimental
mechanical vaginitis in female rats.
Inflammatory infectious
diseases of female genital organs take a special place in the structure of
women morbidity and make up a grate per cent (to 75%) of the total number of
gynecological patients. The most widely-spread pathologies are vaginitis, the
symptoms of which can be found in every third woman of childbearing age. The main clinical
symptoms of vaginitis: swelling, redness of mucous membrane of the vagina,
profuse discharge, nagging pain in a lower abdomen, burning feeling, itching of
genitals.
The factors that affect the vaginitis occurence
are hypofunction of ovaries, insufficiency of thyroid gland, gastro-intestynal
system and other hormonal disorders, microorganisms invation, mechanical
demages of vaginesꞌ mucous membrane (after abortion, after aggressive
methods of treatment, such as criodestruction, electrocoagulation, lazer
therapy etc), violation of personal hygiene rules. In spite of a great number
of factors provoking vaginitis, the treatment should be complex and include antibacterial,
anti-inflammatory, reparative, generaltonic therapy. Local treatment such as bathing and
vaginal suppositories with different medicines is also widely used. The majority of drugs
for this pathology correction at modern pharmaceutical market are synthetic
origin medicines. That’s why, the plant origin drugs are of such great interest
due to their low toxicity and availability of raw materials. Low toxicity also allows to use herbal drugs
for a long period of time without the risk of serious complications taking into
account the childbearing age of
the patients with vaginitis. In addition, the herbal drugs are rich in
pharmacodynamics that allow simultaneously affect the several pathogenetic
links of the disease. But nowdays the number of plant origin medicines for
vaginitis treatment is limited. One of the medicinal plants with potential anti-inflammatory and
reparative properties is the Humulus lupulus extract. The analysis of
phytochemical composition of the given medicinal raw materials indicates the
presence of polyphenolic compounds (flavonoids: kempferol, rutin, quercetin,
xanthohumol), bitter, phytoestrogens. It is also proved that the HL extract
have anti-inflammatory, antioxidant, analgesic and reparative action which can
be used in vaginitis treatment.
That is why the aim
of our research is to observe the influence of the extract Humulus lupulus suppositories on pathogenesis of
experimental «mechanical» vaginitis in female rats.
The model was conducting using special device – scarificator of
vaginasꞌ mucous membrane. Scarification of vaginasꞌ mucous
membrane was conducted under barbamil anesthesia during 5 days. On the 6th day
the treatment of the extract Humulus lupulus suppositories in dose 5 mg/kg and reference drug - suppositories with sea
buckthorn oil in dose 17,64 mg/kg
started. On the 11th day the animals were taken out of the experiment and the
influence of the extract Humulus lupulus suppositories compared with the suppositories of sea-buckthorn oil on the
state of vagina mucous membrane and organism in general was estimated. The following criteria were
taken into account: the general condition of the animals, the condition of the
vagina mucous membrane, which was studied on the 11th day after scarification.
Semiquantitative assessment of inflammation signes was conducted according to
the following 3 parametres: edema, hyperemia and hemorrages. The clinical blood
of experimental rats also was studied.
The results indicate that the mechanical damage of the ratsꞌ vagina mucous
membrane for
5 days leads to the development of vaginitis. The inflammation symptoms of the vagina mucous
membrane of
the
animals
control pathology group manifested a marked soft tissue swelling around the
vagina. The
macroscopic
examination of the vagina mucous membrane of the control pathology group on the 11th day of the
experiment also confirmed the development of an experimental
"mechanical" vaginitis: the affected area of the vagina was 160,38 mm² or
63,37% of the total area, swelling, congestion and numerous hemorrhages were
reflected in the integral indicator – the sum of all the characteristics and
amounted to 7.13 points.
The analysis of macroscopic
morphological studies of vagina mucous membrane on the 11th day also confirms the apparent
therapeutic effect of the extract Humulus lupulus suppositories on the development of vaginitis. This is
evidenced by reliable compared with the control group pathology a decrease in
the affected area of the vagina in rats under the influence of the extract Humulus lupulus suppositories in 3 times, and under the
influence of suppositories with sea-buckthorn oil – only in 2 times.
The local inflammation in
"mechanical" experimental vaginitis was depressed under the
influence of the extract
Humulus lupulus suppositories and
sea-buckthorn oil. The intensity of edema,
hyperemia and hemorrhages in the number of the integral indicator of the state
of vagina
mucous membrane – sum of points, under the influence of the extract Humulus lupulus suppositories – reduced in 2.5 times, and with sea
buckthorn oil - in 2.1 times.
Therefore, the data obtained
indicate the advantage of the therapeutic effect of the extract Humulus lupulus suppositories at a dose 5
mg/kg over a therapeutic effect of the suppositories with sea buckthorn oil at
a dose 17,64 mg/kg in
1.5 times.
The influence of the extract Humulus lupulus suppositories
on the course of experimental "mechanical" vaginitis in rats had
significantly positive character, preventing the formation of pathological changes of
inflammatory changes in vagina
mucose membrane, and reducing the signs of acute inflammation in the case of formed pathology.
Thus, the studies have shown that the extract HL suppositories at a dose of 5 mg/kg, have an apparent therapeutic effect in
terms of "mechanical" vaginitis in rats, and the severity of its
therapeutic effects are superior the drug of comparison – suppositories with sea buckthorn oil at a
dose of 17,64 mg/kg.
Thus, we can
conclude the feasibility of further more in-depth studies of the extract
Humulus lupulus suppositories with
aim to create on their basis a new drug for the treatment of vaginitis.