Medicine / 6. Experimental and clinical pharmacology
Cand. of med. sci. Banzaraksheev V.G.
Institute of
General and Experimental Biology Siberian Department of Russian Academy of
Sciences, Ulan-Ude, Russia
THE RESULTS OF
EXPERIMENTAL STUDY OF MULTICOMPONENT PHYTOREMEDY OF TRADITIONAL MEDICINE
Introduction.
The traditional medicine the special attention is given to use of
multicomponent plant remedies in pharmacotherapy of various diseases [2;7].Complex
plant remedies surely have some advantages over one-component preparations. In
particular, they have versatile effect on the body due to the complex and
balanced chemical composition and rational combination of biologically active
substances. From the one hand they directly influence in the seat of injury,
from the other hand they provide pharmacological correction of various
functional systems, as well as they increase the resistance of the whole body.
Besides, the plants when used in the collection manifest synergism, which
strengthens useful properties of ingredients included in their composition. In
contrast to xenobiotics biologically active substances of various chemical
groups contained in the plants provide not only their polyvalent effect and
complex influence but also a maximal biological accessibility [5]. These
properties provide more gentle effect, high efficiency, good tolerance and
absence of side effects when plant preparations are used for a long time.
The
aim of the research:
Study of anti-inflammatory and immune modulating properties of the
complex plant remedy.
Materials
and methods:
The object of the study was the complex plant remedy,
composed on the base of formulae from the “Chzgud-shi” [2]. The tested remedy in the form of decoction was
introduced intragastrically in the dose of 1 ml/100 g of the weight once a day
during the whole period of the experiment. The control group of animals
received the same volume of the distilled water.
The experiments were carried out in Wistar rats with the initial weight
of 170-190g and male mice of CBA genetic strain with the initial weight of
18-20 g. The influence of the plant remedy in the proliferative phase of
inflammation was estimated taking into account the level of fibrous-granulated
tissue formation. The influence of the plant remedy in the processes of
alteration was studied estimating the area of necrotic tissue [6].
The state of the cell chain of the immune response was studied
estimating the reaction of delayed hypersensitivity on the background of
asathioprine immune deficiency induced by sensibilization of the mice with
sheep erythrocytes.Humoral chain of the immune response was estimated taking
into account the number of antibody-producing cells [2].The obtained data were
processed using Student’s t-criterion [6].
Results of research:
The research on the anti-inflammatory properties of
the plant remedy has established that the tested remedy has a marked
anti-exudative activity, suppressing the development of formalin-induced edema
by 53 % as compared with the data in the control.
The
study of the influence of the tested remedy on the proliferative processes in
inflammation revealed that the mass of dry granulomas was 6 % more than in rats
of the control group. Therefore we suggest that the tested remedy has no
stimulating effect on the proliferation processes in the seat of inflammation.
The
close interrelation of the inflammation process and immune system caused the
need for further studies. The use of the tested plant remedy increased the
index of the reaction of delayed hypersensitivity by 54 % in mice receiving the
remedy on the background of immune suppression as compared with the control.
The introduction of the tested plant remedy on the background of immune
suppression was followed by 1,8 and 1,4 times increase in the number of
antibody-producing cells in absolute means as well as in terms of 106
splenocytes respectively as compared with the data in the animals of the
control group.
Conclusion:
1)The
study of the pharmacological activity of the complex plant remedy indicates
that it has anti-inflammatory and immune modulating effect;
2)The
revealed pharmacological activity of the multicomponent plant remedy is
attributable to the complex of components, which supplement and stabilize each
other, such as flavonoids, tannins, polysacharides and other;
3)The
favorable combination of the given pharmacological properties in the plant
remedy allows to use it with anti-inflammatory as well as with immune
modulating purpose in chronic inflammations, against infections and for
increase of the general resistance of the body.
References:
1.Chzgud – shi: canon of Tibetan Medicine /
Translation from Tibetan, introduction, notes, indicants of Dashiev D.B. – Moscow, 2001. – 766 p.
2.Cunningam A.J. A method of increased
sensitivity for detecting single antibodyforming cells // Nature. – 1965. - ¹
5. – Ð.1106-1107.
3.Managment of experimental study of new pharmacological
substances. – Moscow, 2005. – 832 p.
4.Kumpan-Dudzi: the large reception
dictionary from the Aginsky Datsan / Transl.
from Tibet of Dashiev D.B. – Moscow, 2008. - 216 p.
5.Nikolaev S.M. Multicomponent medical
means of traditional medicine as regulating pharmacological systems //
Baikalskie chteniya-3. – Sankt-Peterburg, 2008. – P. 140-142.
6. Sergienko V.I., Bondareva I.B. Mathematical statistics in clinical
research. – Moscow, 2001. – 256 ð.