Kondratyuk R.V., Trivailo M.S., Saverchenko V.G., shapharenko N.V.

National Technical University of Ukraine «KPI»

THE Unit for cultivation microorganisms

 

The model relates to the microbiological industry, namely, the cultivation of industrial plants to microorganisms in liquid media and can be used for the production of biologically active compounds and vaccines.

Known unit for cultivation microorganisms (UCM), which contains the frame and the platform rotating in an inclined plane with the vessels for cultivation, and the drive (see AS USSR  1731801, C 12 M 1 / 10, 1992).

The disadvantage of known UCM consists in inconvenience of the use, as needs at the use in packing of working liquid.

The nearest to the utility model after technical essence and reached effect accepted for the prototype of UCM, which contains united between itself in underbodies by a flexible pipeline two cylinder chambers with the technological union couplings and devices for serving and taking of exhaust sterile air, and also mechanism of the recurrent-forward moving of chambers in vertical a plane (see, A.s. USSR ¹1131899, C 12 M 1/00, 1984 fig. 3).

The disadvantage of known UCM consists in that its chambers have megascopic weight (resource-demanding) and way of moving, as a result the charges of energy grow on cultivation, and it results in the increase of prime price of product.

The basis of the utility model a task to improve UCM, which means change of chambers is achieved by reducing their weight and the desired movement, which reduces the cost of energy (the movement of chambers) and leads to lowering the cost of use.

This task is solved with UCM, which contains united between itself in an underbody by a flexible pipeline two chambers with the technological union couplings and devices for a serve sterile and taking of exhaust air, and also mechanism of the recurrent-forward moving of chambers, in vertical a plane, in obedience to an useful model, new is that chambers have a form of spheres.

Marked a distinctive sign provides, as compared to a prototype, diminishing of weight and necessary moving of chambers, which decreases expenses energy on their moving and brings the use over to the decline of cost. Except for it the sizes of UCM diminish on its height.

Claimed UCM is schematically represented on a draft, general view.

 

UCM contains united between itself a flexible pipeline 1 two chambers 2, 3 with devices 4, 5 for the serve of sterile air 6 and taking exhaust. Chambers 2, 3 have a spherical form of radius of «R» and added to the mechanism them irregular recurrent-forward moving in a vertical plane, the occasion of which is contained by a reversible reducing motor-gear 7 with control block 8 and by a drum 9, which engulfs thrown over through blocks 10 and added ends to the chambers rope 11. Cameras 2, 3 have technological connections 12 for a fluid and tube 13 to connect a flexible pipe 1. Flexible tubing 1 has a valve 14 for coacervating content cameras and technological ceiling of pipeline.

UCM works as follows.

One of the pre sterilized chambers, such as shown in fig. 1 the camera position, fill to the required level of life-giving liquid H and planting material (working fluid). Then open the valve 14 in pipeline 1 and carry out cultivation placed in nutritional liquid cultures. Open the tap 14 pipe 1 leads to the flow of fluid through a pipeline 1 with the camera 2 to camera 3, displacing it from the air 6. When flow is mixing fluid and increase its level of H1 in the chamber 3. After filling the fluid chamber to the desired level 3, control unit 8 switches the motor reducer 7 and change camera position in the vertical plane: the camera 2 lead in the bottom position, and camera 3 - in the top position, where they remain for a specified time for transfusion.

Overflow fluid between the chambers in a different situation occurs in the opposite direction, that from the camera 3 in the chamber, 2 previously described method.

After the required number of cycles of displacement and time transfusion contents poured into the chamber through valve 14 for further use.

So as spherical chambers as compared to cylinder in a prototype at the identical thickness of walls and volume have considerably less weight and need the diminished way of moving (as a result of less size on a height), charges of energy on the multicyclic moving of chambers in the process of cultivation diminish substantially, and it brings the use over to lowering of cost, the also diminished height of UCM is instrumental in it.