Медицина/6. Экспериментальная и клиническая фармакология

Zhukov S.V.², Lebedev K.A. ², Kaplin A.N.², Full Prof. Pokrovsky M.V.¹, MD Pokrovskaya T.G.¹, PhD Danilenko L.M.¹, MD Korokin M.V.¹, MD Kochkarov V.I.¹, PhD Korokina L.V.¹, PhD Gudyrev O.S.¹, PhD Arustamova A.A.¹

¹Federal State Autonomous Institution of Higher Professional Education "Belgorod State National Research University", Russia

²State Educational Institution of Higher Professional Education "Kursk State Medical University", Ministry of Health, Russia

Modeling of ischemic and pharmacological preconditioning on isolated rat’s heart

One of the methods to study the effects on the organ level are experiments on isolated organs, including isolated by Langendorff rat’s heart. Described in 1986 S.Murry and co-authors myocardial ischemic preconditioning phenomenon is manifested by increased resistance to myocardial ischemia after one or more short ischemic episodes and lasts for 1-2 hours [1, 5, 6].

The aim of this study is to investigate the phenomenon of ischemic preconditioning and pharmacological preconditioning with recombinant erythropoietin on isolated rat heart by Langendorff.

The study was performed on 30 rats of both sexes, weighing 200-300 g aged 3-4 months, Wistar, contained in a 12-hour light day on a standard diet c briquetted food, with free access to food and water. The animals were divided into 4 groups (10 rats in each group): Group 1 - rats with ischemia, 2nd - rats with preconditioning, third pharmacological preconditioning with recombinant erythropoietin 50 ED/L, 4th control. In rats under ether anesthesia, opened his chest, removed his heart. In the opening aortic cannula was inserted and started to pass through it heated to 37-39 ° C aerated Krebs-Henzeleyta. After the experiments observed reduction of isolated heart for 2 hours. After this time received 4 slice from each heart and placed in a solution of triphenyl tetrazolium chloride (TTC) for 15 minutes in an incubator at a temperature of 37,5° C. TTC solution stains living tissue with preserved lactate dehydrogenase activity, and ischemic tissue has lost enzymatic activity does not stain. Stained sections were photographed and then processed using Photoshop.

Modeling of the 40-minute ischemia followed by reperfusion resulted in progress of myocardial tissue damage, the area of which amounted to 62,5 ± 1,3%. In the second group, the modeling of ischemic preconditioning, the area of damage was 31,8 ± 2,5%. Pharmacological preconditioning with recombinant erythropoietin showed comparable protective effect of ischemic reconditioning to the area and was 30,1 ± 2,4%. Increase of concentration till 500 ED/L did not lead to enlarge the protective effect, and the reduction up to 25 ED/L caused withdrawal of cardioprotective effect. In the control group, the myocardial tissue injury was not revealed.

Thus, the modeling of ischemic and pharmacological preconditioning on isolated rat’s heart by Langendorff has cardioprotective effects on myocardial tissue, which allows to use the model for experimental analysis of the mechanisms of preconditioning.

The study was supported by a grant of the President of the Russian Federation

№ MK-905.2012.4.

The study was conducted as part of the state task for R & D (State Contract № 4.913.2011).

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