Severinova S. K., Bobkova S.A, Makarenko V.N.

Crimea state medical university named after S. I. Georgievsky, Simferopol.

Influence bridges based on implants on cytochemical indicators on the state of peripheral blood neutrophils

In dental implantology despite of significant progress and scientific achievements, as before, remain relevant issues related to the prevention of inflammatory complications in tissue complex prosthetic support zones.

Cytochemical studies were used to assess the activity of dehydrogenases of peripheral blood neutrophils, activity of LDH and LDH.The data obtained in the study of the treatment of orthopedic patients with bridges relying on implants can be the basis for the possibility of reducing the likelihood of complications.The aim of our study was to conduct monitoring cytochemical peripheral blood neutrophils in orthopedic patients after installing bridges implant.

Methods of investigation

Pictures of our study served as peripheral blood of patients who underwent dental implantation . To conduct the study were examined 26 people of both sexes , requiring orthopedic treatment with bridges .Cytochemical methods of research dehydrogenase activity in peripheral blood neutrophils .Certain interest to study the activity of dehydrogenase enzymes of glycolysis and the Krebs cycle . Quadrupeds that succinate dehydrogenase and lactate dehydrogenase is one of the most important cellular enzymes.

Dehydrogenase - enzymes that cleave hydrogen from an appropriate substrate and transfer it to the acceptor . This mechanism of action used in cytochemical methods , which are introduced into the system indicator assumes hydrogen. The indicator thus should be of a color and precipitate. For these purposes, the most commonly used water-soluble tetrazolium salt : neotetrazolny chloride ( NT ), blue tetrazolium ( PT) nitrotetrazolny blue (NBT ) and others that under the action of reducing agents are converted into water-soluble colored formazan , allowing you to install intracellular localization of dehydrogenases under a light microscope.

Method for studying the activity of dehydrogenases in blood neutrophils proposed by us when cells of heparinized blood smears made after treatment with appropriate reagents hour after incubation.In studies as an indicator enzyme process used nitgrogetrazoly blue ( NTS ) , which forms the reduction in cell fine granules of formazan staining cytoplasm of gray to deep blue. We used native thin blood smears , air-dried , which, after appropriate processing . Incubated for 45 minutes at 37 ° C. Cell nuclei dokrashivali solution Methylene green . Dried smears microscope with immersion lens microscope MBI- 15.To determine the activity of LDH apply solutions : sodium cyanide , sodium lactate , NST . gemodez , NAD . Deposition granules formazan observed at the location of EDC . Staining intensity ranged from smoky gray to intense blue , cell nuclei were well circumscribed .To determine the activity of LDH - solutions : NBT , succinate Katra , phosphate buffer . There was a clear localization of formazan granules . The activity of LDH was judged by the intensity of formazan deposits granules . To assess the activity of enzymes in blood cells , we calculated the average cytochemical ( SCP ) .

Cytochemical study of peripheral blood neutrophils .In the analysis of the cytochemical activity of peripheral blood neutrophils in patients with orthopedic established with bridges revealed :By 1 month after prosthesis wearing light and insignificant character ( P> 0.05 ) , enzyme imbalance , so the activity of succinate dehydrogenase ( LDH ) were 1 , B7 ± 0,06 conv , which was 9.2% below control and enzymatic activity of lactate dehydrogenase (LDH ) 2,48 ± 0,16 conv that was by 7.8 % higher than control Are .To 3 months after prosthesis with bridges imbalance decreased since LDH activity was 1,72 ± 0,09 conv , which was 6.8% below koshrolnyh indicators, and LDH activity was 2,41 ± 0,11 conv. u , which was 4.7% higher than the control ( P > 0.05).To 6 - 12month , we were treated as long-term results , cytochemical activity of peripheral blood did not differ from controls.

Conclusions

Detailed analysis of cytobiochemicical studies allowed to conclude that the installation of bridges (made of acrylates ) implant has no significant changes in the state of neutrophils in the peripheral blood of patients , as evidenced by indicators dehydrogenase activity of peripheral blood neutrophils and LDH and LDH .