Dvorshchenko
K.O., Borodina T.V., Ostapchenko L.I.
Taras Shevchenko Kyiv National
University, Kyiv, Ukraine
Influence
of multiprobiotic "Symbiter" on the content of neutral and phospholipids
in rat liver and pancreas during
long-term gastric hypochlorhydria
Pathological conditions of the gastrointestinal tract,
which are accompanied by reduced synthesis of hydrochloric acid, lead to
morpho-functional and microecological violations in the gastrointestinal tract,
particularly in the liver and pancreas [1, 2]. There is evidence of connection between the
development of pathological process and damage of biomembranes lipid layers by
reactive oxygen species [3].
To restore the oxidative-antioxidant balance it is
necessary to replenish stores of intracellular antioxidants. It is known that
probiotics have antioxidant properties [4]. We used multiprobiotic
"Symbiter® acidophilic" concentrated (“Symbiter”) – the concentrated biomass of living
cells of multicomponent symbiotic probiotic bacteria (Bifidobacterium, Lactobacillus,
lactic streptococci and propionic acid bacteria).
The aim of the study was to
determine the content of neutral and phospholipids in rat liver and
pancreas in conditions of long-term hypoacidity, evoked by omeprazole
and the effect of multiprobiotic “Symbiter” on this process.
Materials and
methods. Experiments were carried out on white non-strain male rats with initial
weight around 180-200 g. All animals were divided into four groups. Rats
injected abdominally with 0,2 ml of physiological solution and 0,5 ml of water
for injections orally were used as a control (first group). Animals of second
group were treated with the same dose of multiprobiotic “Symbiter” orally (0,14 ml/kg) during 28
days. Hypoacidity (third group) was modeled by everyday intraperitoneal
injection of omeprazole (14 mg/kg) during 28 days. Fourth experimental group
simultaneously with omeprazole obtained multiprobiotic “Symbiter” orally (0,14 ml/kg). Number
of animals in each experimental group was 10.
Lipids from rat liver and pancreas were extracted using the Folch method by chloroform-methanol mixture (2:1, v/v) [5]. Lipids composition was determined by
thin layer chromatography
analysis. Statistical processing of
experimental data was performed with analysis of varience. Probability of
difference between control and test measurements was assessed with Student’s
t-test.
Results
and discussion. It was shown that long-term inhibition of gastric secretion of
hydrochloric acid by omeprazole caused significant changes in the structural
organization of rat liver and pancreas lipids.
In the liver tissue, we observed
increased content of free fatty acids - for 2,8 times; of cholesterol esters - for 1,5 times in comparison
with control. Under these same conditions we fixed decrease of cholesterol and triacylglycerols for 1,9 and 1,3
times, respectively. At the same time, upon long-term gastric hypoacidity we fixed increased content of lysophosphatidylcholine - for 1,9 times and
content of lysophosphatidylethanolamine - for 2 times in
comparison with control. It was determined that
prolonged influence of omeprazole on rat liver decreased content of phosphatidylcholine – for 1,3 times, phosphatidylethanolamine – for 1,4 times, phosphatidylserine – for 1,8 times and
phosphatidylinositol
– for
2 times, respectively in comparison to the control group of animals.
In the pancreas tissue, it was shown that long-term
inhibition of gastric secretion of hydrochloric acid by omeprazole caused
increased content of free fatty acids - for 1,6 times; of cholesterol esters - for 1,4 times in comparison
with control. Under these same conditions we fixed decrease of cholesterol and triacylglycerols for 1,5 and 1,3
times, respectively. At the same time, upon long-term gastric hypoacidity we
fixed increased content of phosphatidylinositol – for 2,4 times, lysophosphatidylcholine - for 1,7 times and
lysophosphatidylethanolamine - for 2,4 times in
comparison with control. Study in the content of lipid in rat pancreas showed that after
prolonged supression of secretion of hydrochloric acid their level decreased: phosphatidylcholine– for 2,2 times, phosphatidylethanolamine – for 1,4 times and
phosphatidylserine
– for 2,6
times, respectively.
At combined
introduction of animal multiprobiotic “Symbiter” with omeprazole, we fixed significant
restoration of content of liver and pancreas lipids compared with that in group
of rats with hypoacidity state.
Thus, our findings suggest that long-term
gastric hypochlorhydria is
characterized by changes in the composition and the rate of oxidative changes
in membrane lipids of rat liver and pancreas. Administration
of “multiprobiotic “Symbiter” to the rats with gastric hypoacidity was associated with the
normalization of investigated parameters in liver
and pancreas tissue via
normalization microbiota in the gastrointestinal tract.
Literature.
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