Nepreluk O.A., Bobkova S.A., Severinova S.K.

Crimea State Medical University named after S. I. Georgievsky

"Impact of prosthesis fixed structures on the biochemical parameters of mixed saliva"

At present, it is safe to say that the method of dental implantation has taken its rightful place among other dental procedures. In most cases, the implants are made from titanium. With the growth of allergic reactions to various metals and metal alloys used in dentistry, titanium regarded as a crucial alternative. Thanks to the wonderful and incredible stability, biocompatibility of titanium, this metal has attracted the attention of orthopedics. . Due to that it does not corrode and does not give the free metal ions which are capable of surrounding the implant or prosthesis to cause pathological processes. But, in spite of the unique qualities, we cannot exclude the development of adverse electrochemical processes using dental alloys in fixed prosthesis with reliance on titanium implants. Implantation is often accompanied by various complications of the soft tissue and bone, these effects lead to inflammatory and atrophic processes, in which the leading role played by the materials of construction of prostheses and implants.

Priority and promising area of medicine is the development of new technologies increases the functional reserves of a healthy person, it is important for the rehabilitation of patients with surgical interventions. The aim of our study is to determine the level of acid-stable inhibitors of mixed saliva orthopedic patients as a criterion for assessing the compatibility of implant materials and various types of fixed prostheses, prosthetic

adaptation processes in various types of fixed restorations to implant.

A change in their balance sheets is a typical manifestation of the body's response to injury at the molecular, cellular and systemic levels.

To determine the level of acid-stable inhibitors (CSIs) in saliva sample was pretreated for depositing acid-labile proteins. For this 1 ml of saliva was mixed with 1 ml of 0.05 M Na-acetate buffer (pH 1.4) and heated in a water bath at 60 º C for 20 minutes. To determine the CSI in temperature-controlled spectrophotometer cuvettes prepared two samples - experimental and control. Experimental sample contained 1.4 mL of Tris-HCl buffer (pH -0,08). 0.5 ml of saliva, and 0.1 ml trypsin (10 mg) in 1 mM BAEE solution was stirred rapidly and increase the optical density measured at 253 nm against the sample by spontaneous hydrolysis of the substrate. Counts were doing every minute for 4 -5 minutes from the linear portion of the curve depending on the optical density of growth for 1 minute for the test and control samples. The difference between these values is used to calculate the CSI in IU / ml. To conduct the study were examined 38 people (22 women and 16 men) aged 21-40 years. By selecting patients, we take into account the overall health, past illnesses anatomical and physiological features of the oral cavity. Patients were divided into 2 groups: Group 1 included 20 patients who, after implantation were fixed metal-ceramic prostheses based on cobalt chrome alloy. The second group - 18 people have been attributed to patients who are in prosthetics were made without Metallic ceramic prostheses implant. Each group was allocated to 2 subgroups: subgroup A - without any additional therapeutic interventions, subgroup B - patients after fixation of permanent structures in the daily \ m into the gluteal muscle was administered the drug "Erbisol" 1 ml course of 10 days. Also examined 15 healthy individuals not suffering from dental pathology - a control group of Orthopedics treatment was carried out by two staged procedure endosseous implant screw implants "Uimpl" with further prosthetics different fixed structures. In conducting the study, we used the drug "Erbisol" - an immunomodulation drug and adaptive. Erbisol class drugs affect only the unbalanced system, damaged organs and tissues and are almost indifferent to the healthy organism without causing adverse reactions. Processing of the results of research performed with the use of standard software package Microsoft Office 2000 on a PC-class Pentium.

Analysis of the indicators of acid-inhibitors (CSI) of the mixed saliva of orthopedic patients with different types of fixed prosthesis implant-supported by 1 month of observations in all groups showed a statistically non-significant observation changes. In the following 2 months of observation there is a progressive increase in the CSI in both groups.

In the subsequent period of observation (5-6months) indicators of acid-stable inhibitors of mixed saliva orthopedic patients in the two groups of observations approaching targets, acquiring statistically insignificant nature in relation to the control.

The study indicators of CSI mixed saliva orthopedic patients with different types of fixed prosthesis implant-supported been an increase performance regardless of the type of prosthesis to 3 months after the prosthesis. The fastest recovery indicators CSI observed in Group 2, where the used metal-free ceramic prosthesis - to 4 months of observation. The use of an immunomodulation and adaptogen "Erbisol" contributed to a more rapid normalization of the level of CSI: in group 1, which used metal-ceramic prostheses - to 5 months, in group 2 were used where metal-free ceramic prosthesis - to 3 months.