BOD-biosensor based yeast Debaryamyces
hansenii VKM Y-2482 encapsulated in organosilicate matrix
Afonina E.L, Kamanina O.A Ponamoreva
O.N
Tula State University
At present special
attention is paid to the purification of natural and household water from various
types of pollution. The standard method of determining the biochemical oxygen
demand (BOD) requires incubation oxygenated sample for 5 or 20 days. Lack of
efficiency significantly reduces the value of such a definition. The standard
method of determining the biochemical oxygen demand (BOD) requires incubation
oxygenated sample for 5 or 20 days. Lack of timeliness significantly reduces
the value of such a definition. For this reason, there may be really
environmentally hazardous situations in which remains a "behind the
scenes" of contaminated water in the reservoirs. Modern strict
requirements of environmental conditions dictate the need to use rapid methods
of monitoring the BOD. For operational analysis developed method of estimation
BOD, based on the use of biosensor analyzers to reduce the time of analysis of
700 times. Immobilization of microorganisms is an important and essential step
in creating a biosensor.
For the immobilization
of whole cells suggested a variety of approaches and methods each of which has
its advantages and limitations. Special interest represents immobilization of
biomaterial hybrid organosilicate matrix, which have a number of advantages
over organic and inorganic polymers: long preservation at a high level of
biological activity of the microorganisms after immobilization process; provide
effective protection from external, damaging the structure of the matrix
factors such as mechanical, thermal and biological effects; a constant volume
and high porosity matrix regardless of the composition of the medium, which
provides high-speed diffusion fluxes, as well as they are capable of safely and
efficiently keep the biomaterial. In the work microorganisms were immobilized
using this technology and used as a biocatalyst in a biosensor for the
determination of BOD. One of the most promising microorganisms for the creation
of BOD biosensor is yeast Debaryamyces
hansenii VKM Y-2482, which have a broad substrate specificity.
Immobilization these yeast organosilicate matrix, allowed to obtain a receptor
element of the biosensor characterized by a heightened sensitivity compared to
other methods of immobilization. [1]
For the purpose of
testing and calibration correlative obtained biosensor was determined BOD in
model samples of water from the water sources of the Tula region (Russian
Federation). As a reference method used a standard method of determining BOD.
Statistical analysis of the results of BOD showed that the samples obtained by
the two methods are uniform in reproducibility. Biosensors based on
encapsulated yeast Debaryamyces hansenii
VKM Y-2482 in a silicone matrix is a perspective tool for monitoring pollution
of natural and household water.
1. V.A. Arlyapov, N.Yu. Yudina, L.D. Asulyan,
S.V. Alferov, V.A. Alferov, A.N. Reshetilov BOD biosensor based on the yeast
Debaryomyces hansenii immobilized inpoly(vinyl alcohol) modified by
N-vinylpyrrolidone // Enzyme and Microbial Technology – 2013 – ÅÌÒ 8518
The work was supported by the Federal Target
Program "Research and development on priority directions of
scientific-technological complex of Russia for 2014 - 2020 years", the
agreement ¹ 14.574.21.0062