Dvorshchenko K.O., Borodina T.V., Ostapchenko L.I.

Taras Shevchenko Kyiv National University, Kyiv, Ukraine

Influence of multiprobiotic "Symbiter" on the content of adenine nucleotides in rat liver and pancreas during long-term gastric hypochlorhydria

Multiprobiotic "Symbiter® acidophilic" concentrated (“Symbiter”) thanks to its high antibacterial, immunomodulatory and antioxidant properties have established itself as a prophylactic and therapeutic agent for pathologies of the gastrointestinal tract [1]. Long-term use of blockers of gastric secretion HCl causes the development of dysbiosis and inflammatory processes in the gastro-intestinal tract [2].

Gastric hypoacidity leads to pathological changes in liver and pancreas cells, one indicator of which is the imbalance of adenine nucleotides. Adenine nucleotides play an important role in intermediate metabolism in cell, forming parts of coupling between processes which use and generate energy. Normalization of energy status of cell is the necessary condition for regeneration of liver and pancreas at the given pathology.

The aim of our study was to determine the effect of multiprobiotic “Symbiter” on the content of adenine nucleotides in rat liver and pancreas in conditions of long-term suppression of secretion of hydrochloric acid.

Materials and methods. Experiments were carried out on white non-strain male rats with initial weight around 180-200 g. All animals were divided into three groups. Rats injected abdominally with 0,2 ml of physiological solution and 0,5 ml of water for injections orally were used as a control (first group). Hypoacidity (second group) was modeled by everyday intraperitoneal injection of omeprazole (14 mg/kg) during 28 days. Third experimental group simultaneously with omeprazole obtained multiprobiotic “Symbiter” orally (0,14 ml/kg). Number of animals in each experimental group was 10.

Separation and quantitative definition of the content of adenine nucleotides in organs was carried out by method of thin-layer chromatography and direct densitometry of silufol plates in UV-light. Statistical significance in each group was tested using one-way analysis of variance with individual means detected by the Student test. All data are presented as mean ± standard error, and a p value less than 0.05 was considered significant.

Results and discussion. It was shown that long-term inhibition of hydrochloric acid secretion by omeprazole caused changes in the content of adenine nucleotides in rat liver and pancreas.

Cellular ATP concentrations decreased significantly during long-term gastric hypochlorhydria (Tables 1, 2). Liver ATP dropped from 2,61 ± 0,26 to 1,59 ± 0,15 micromol/g dry tissue (39%), and pancreas ATP from 1,28 ± 0,12 to 0,61 ± 0,06 (52%).

In the liver tissue, we observed increased content of ADP – for 1,4 times and of AMP – for 1,3 times in comparison with control group of animals (Table 1).

Table 1.

The content of adenine nucleotides in rat liver during long-term gastric hypochlorhydria, (M ± m, n = 10).

Content of adenine nucleotides Group of animals	ATP, micromol x g tissue^-1	ADP, micromol x g tissue^-1	AMP, micromol x g tissue^-1
Control	2,61 ± 0,26	0,93 ± 0,09	0,38 ± 0,03
Omeprazole	1,59 ± 0,15^*	1,28 ± 0,12^*	0,51 ± 0,05^*
Omeprazole + “Symbiter”	2,18 ± 0,21^*/#	1,13 ± 0,11^*/#	0,45 ± 0,04^#

*- P ≤ 0,05 in comparison to control;

# - P ≤ 0,05 as compared to the group of animals injected with omeprazole.

In the pancreas tissue, it was shown that long-term inhibition of gastric secretion of hydrochloric acid by omeprazole caused increased content of ADP – for 1,7 times and of AMP – for 1,5 times in comparison with control (Table 2).

Table 2.

The content of adenine nucleotides in rat pancreas during long-term gastric hypochlorhydria, (M ± m, n = 10).

Content of adenine nucleotides Group of animals	ATP, micromol x g tissue^-1	ADP, micromol x g tissue^-1	AMP, micromol x g tissue^-1
Control	1,28 ± 0,12	0,46 ± 0,04	0,19 ± 0,02
Omeprazole	0,61 ± 0,06^*	0,78 ± 0,07^*	0,28 ± 0,02^*
Omeprazole + “Symbiter”	0,91 ± 0,09^*/#	0,59 ± 0,05^*/#	0,24 ± 0,02^#

*- P ≤ 0,05 in comparison to control;

# - P ≤ 0,05 as compared to the group of animals injected with omeprazole.

At simultaneous injection to rats of multiprobiotic “Symbiter” and omeprazole, we fixed significant restoration of adenine nucleotides content in the liver and pancreas tissues compared with that in group of animals with gastric hypoacidity.

Thus, our findings suggest that long-term gastric hypochlorhydria is characterized by changes in the content of adenine nucleotides in rat liver and pancreas, that testifies to infringement of energy state of these organs. Administration of multiprobiotic “Symbiter” to the rats with hypoacidity state was associated with the restoration of energy balance in liver and pancreas tissues.

Literature.

1. Dvorshchenko K.O., Senin S.A., Ostapchenko L.I. Influence of multiprobiotic “Simbiter acidophilic concentrated” on pro- and antioxidant balance in rat liver during long-term gastric hypoacidity // “ANNALES UMCS, sectio Pharmacia”. – 2010. –Vol. XXIII, №1. – P. 161–165.

2. Canani R., Terrin G. Gastric acidity inhibitors and the risk of intestinal infections // Curr. Opin. Gastroenterol. – 2010. – Vol. 26, №1. – P. 31–35.