Karachun V.V., Trivailo M.S., Mel’nick V.N.

National Technical University of Ukraine “KPI”

THE CULTURING CELLS IN LIQUID MEDIA

The utility model relates to biotechnology and can be used for culturing cells in the production of vaccines and a variety of biologically active drugs.

Known apparatus for culturing cells (DСC), comprising a cylindrical body with technology and Cantilever pipe fixed on the bottom shell reshuffle drive with nonmagnetic materials, and drive in the form set out in the rotor disk and periodically interacting magnets (see for example A.S. USSR №1306944, С 12 М 3/00, 1987).

The disadvantage of this DCC is low efficiency of mixing, due to the console and drive consolidation serves the cause of performance degradation.

The most useful model close to the technical essence and dosyahayemym effect is taken as a prototype of the DCC, which contains the vertical cylindrical body with a lid and technological pipe reshuffle device as a rod with a disc of magnetic material and the drive rod in the form of uniformly arranged in a circle in the bottom case consistently connected to power electromagnets (see., patent of Ukraine for utility model №12120, C 12 M 3 / 00, 2006).

The main disadvantage of the famous DCC is existing damage to cells due to mechanical friction during the cultivation due to the location of support surface mixing device in the volume of fluid, which reduces the quality of cultivation and conformity of the finished product.

The basis of the utility model a task to improve the DCC, which means the location of surface friction, mechanical stirring device outside the working fluid is removed damaged cells cultivated serving quality.

A task is solved that in the DCC, which contains a vertical cylindrical body with a lid and technological pipe reshuffle device as a rod with a disc of magnetic material and the drive rod in the form of uniformly arranged in a circle over nosed drive consistently connected to power electromagnets under the new utility model is that the core hinges attached to the center of the lid, and lid made of nonmagnetic material.

These distinctive features, unlike the prototype, eliminate mechanical friction and thus damage his cell in the process of cultivation that leads to an increase in quality.

The drawings show claimed DCC (fig.1).

Fig. 1.

DCC has vertical cylindrical housing 1 with lid 2 with non-magnetic material and technological pipe 3 to enter and remove cultural (working) fluid 4. In case 1 set reshuffle device type of bar 5 with pressed on it and made of magnetic material disc 6. Bar 5 joint added an overhead end to the center of lid 2, for example, by the spherical tailpress foreseen for this purpose 7 with a holder 8, or by known another way. On a lid 2 the set occasion 9 in form equispaced on a circle above the overhead butt end of disk 6 electromagnets 10, which in the programmatic pulse-mode are connected consistently to the source of feed 11. Except for round, mixing bar 5 can have flat or other known geometrical form.

DCC works as follows.

At including of electromagnetic occasion 9 electromagnets 10 form the rotary-type magnetic field which attracts a disk 6, as a result it comes in a sinewave, and the bar connected with it 5 – in circulating motion about axis of corps 1. Revolved, bar 5 carries out active interfusion of working liquid 4.

Being in suspended to the lid 2 states, in place of benthic location in a prototype, mixing bar 5 and it drive disk 6 does not create the working liquid of 4 areas of mechanical friction in a volume, and that is why inevitable damage them of cages at cultivation, as it takes place in a prototype, and it results in growth of quality.

Except for it, a construction is simplified, as a corps acquires form of one detail, in place of two details in a prototype.