Karachun V.V., Trivailo M.S., Mel’niсk V. N.

National Technical University of Ukraine «KPI»

THE MASS-EXCHANGE AND AERATION IN BIOREAKTOR'S

The utility model belongs to biotechnology and may be used in the microbiological, pharmaceutical and food industries for cultivation of cells, probiotic cultures, the culture of lactobacillus, and for needs of medicine and clinical researches.

The known plant for cultivation of microorganisms, which consists of two chambers, which are connected in the lower part by flexible piping. These chambers have air filters and reversible drive for their reverse-forward movement in the vertical plane (А.c. The USSR №1131899, С12 М1/00, 1984).

The weakness of this plant are the inadmissible big dimensions, which are causing by the necessity of movement of the chambers in the vertical plane.

Also there is known the apparatus for cultivation of cells (AC), which contains a body with the technological union couplings and the hollow shaft, which is placed on the axes of the body of impulsive drive with a blushing, added with the mixing element in form of four-line hinge with shoulder-blades on ends, the filtering element connected with the interstice of the shaft and mobile hinge bush, and also an aerator (A.c. The USSR 1633814, C12 M3/00, 1995).

This AC is the closest to the utility model for it’s technical essence and achivable effect. The weakness of it is the deffisient productivity of making of the product.

The noted weakness is explaining by condition, when the diminishing of number of turns of the shaft, the intensity of mixing and cells of biomass goes down and do not provide in largo manum by oxygen which slows their development. The opposite, with the increasing of number of turns of the shaft, the mixing element inflicts physical harm to microorganisms, limiting the rates of their development which also interferes with growth of the productivity. Except this, a vehicle has an elaborate design which can be considered as another his defect.

The basis of utility model is the task to improve the vehicle for the cultivation of cells by modification of form and structure of the motion of mixing element, and as a result the necessary degree of active mass exchange is providing and diminishing of degree of risk of damage of the shells of microorganisms. Providing of these terms serves to increase the productivity with simultaneous simplification of construction.

The given task is solving by that in АC which contains the cylindrical case with the technological branch pipes, a shaft with the plug placed along an axis of the case which is attached with a mixing element, an aerator, and also a reversive drive, according to utility model there is a new fact of presence of contacting surfaces of a shaft among themselves and the plug in the form of carving connection, and a mixing element - in the form of a disk which is saved from the reference around the vertical guide.

The outlined difference allows to intensify process of hashing of a biomass without damage to the cells which raises productivity, i.e. increases quantity of an exit of probiotics which structure includes live cells of producers. Simultaneously with it the design as the mixing element has simple and reliable in work the geometrical form becomes simpler.

On fig. 1 the offered device for the cultivation of cells is represented schematically.

AC contains the cylindrical case 1 with a branch pipe 2 for introduction of a nutritious liquid and a sowing material, a branch pipe 3 with аthe aerator 4, a branch pipe 5 for removal of the nutrient liquids, and a branch pipe 6 for removal of the fulfilled gas. Along a axis of the case 1 the shaft 9 with the plug 10 is located and is attached to a motor-reducer 7 with the command reversing device 8. On this plug 10 a mixing element –the disk 11 in the form of established with a clearance "δ" concerning the walls of the case 1. The contacting among themselves surfaces of a shaft 9 and plugs 10 are executed in the form of carving connection (screw pair), and the disk 11 counts fixed in the case 1 vertical guide 12.

The device works in the following way. In preliminary sterilized AC to the case 1 enter through a branch pipe 2 nutrient liquid and a sowing material (subculture) then in the aerator 4 gas for aeration of cultural medium arrives. The command device 8 engages, which signal puts in action the motor-reducer 7 and a shaft 9 which within 8 courses set by the command device "Н" gives back and forth motion to the plug 10 and attached to it and saved from the reference to a disk 11guide 12.

Moving along a shaft, the disk 11 would carry out the mass exchange of spilling over biomass through a backlash without the damage to the cells owing to what the conditions for more intensive growth of productivity of process are created.

The absence in the offered construction also assists to reduction of the risk of damage to the cells, and also to a design of rotating movement of a disk 11 that takes place in a prototype, and its simple form leads to design simplification as a whole. Upon termination of cultivation process, the motor-reducer 7 is stopped, and a finished product merge through a branch pipe 5 for further using.