Kondratjuk R.V., Trivailo M.S., shapharenko N.V., Saverchenko V.G.

National Technical University of Ukraine «KPI»

THE PAIR OF DIFFERENT ROTARY WHEES OF SEGNER IN BIOREAKTOR'S

The utility model concerns to biotechnology and can be adapted for realization of processes of microbiological synthesis for the sake of cultivation of aerobic microorganisms in liquid environments.

The known device for cultivation of microorganisms (DCM) which contains the cylindrical case with a thermostatic cover and branch pipes for giving of a nutritious liquid compressed air and removal of a finished product and the fulfilled gases, the device for the aeration and mixing in the form of the wheel of Segner placed in the case located over the bottom with apertures in the walls, and also the device for defoaming (for example, А.c. The USSR № 1349241, С12M1/06, 1985).

The lack of this device consists in its inadmissible low efficiency together with high intensity of the foaming owing to additional expenses of compressed air through apertures in walls of the wheel of Segner.

Known also DCM which contains the cylindrical case with a thermostatic cover and branch pipes for leading of a nutritious liquid with the compressed sterile air and removal of a finished product and the fulfilled gases, the device for aeration and mixing of the cultural liquid in the form of placed on a hollow axis with reference possibility to the opposite sides of two wheels of Segner located co-axis in the emptiness of the case, and also the device for defoaming (for example, the patent of Ukraine for the invention № 54579, С12M1/06, 2003).

As its lack low is efficiency of mixing for insufficient heights of the wheels of Segner which is caused by their rectilinear (flat) form in axial section. This factor essentially assists to the decline of productivity of the device.

The basis of the utility model is the task of improvement of the device in which by form change of the wheels of Segner in axial section, their height which raises intensity of the mass-exchange increases and serves to the productivity growth.

The given task is solving by that in the device which contains the cylindrical case with a thermostatic cover and branch pipes for receipt of a nutritious liquid with the compressed air and tap of a finished product and the born gases, the device for aeration and mixing in the form of two wheels of Segner located on a hollow (tubular) mark with reference possibility in opposite sides which is located in emptiness of the case, and also the device for defoaming, according to the utility model the new there is that the wheels of Segner in axial section have the П-shaped form.

The granting to the wheels of Segner П-shaped forms in axial section increases (in comparison with a prototype) their height, and so and a thickness of layers of the cultural liquid mixed by them, under other equal conditions, intensity of the mass exchange raises and leads to productivity growth.

On fig. 1 a the studied device in axial section are represented schematically, on fig. 1 b– section А-А on fig. 1 a.

The device for cultivation of microorganisms contains the cylindrical case 1 with a lid 2 and a cover 3 for giving in its emptiness of the thermostatic environments (water, steam), and also branch pipes for leading of a nutritious liquid 4 and compressed sterile air 5, and removal of a finished product 6 and created gases 7. The device for the aeration and mixing of the cultural liquid 8 which is executed in the form of placed on coaxial with the case a hollow axis 9 of two wheels of Segner 10, 11 are located in the emptiness of the case 1. The wheels of Segner 10, 11 consist of radial tubes 12 that have nozzles 13 on the ends and connected among themselves free, put on an axis 9 naves 14 with internal ring cavities 15 and fixing rings 16. The axis 9 in an arrangement zone of the cavities 15 of the naves has apertures 17 for passing at a tube of the wheels of the air and giving of air attached to a branch pipe 5. The wheels of Segner 10, 11 in axial section have the П-shaped form “a b c d” that increases their height Н in different diameters D₁, D₂ and an opposite direction (fig. 1.7) of the nozzles 13. On an axis 9 the device for defoaming which is equipped by draughts 18 attached to the top wheel 11 with possibility of axial moving of a float nave (plug) 19 with screw blades 20 is also placed.

Works DCМ in the following way.

After refuelling through a branch pipe 4 of the sterilized case 1 with the nutritious liquid with the seed culture fermenter (the cultural broth), in a branch pipe 5 arrives compressed sterile air and cultivation of the cultures placed in a nutritious liquid is carried out. Thus, air which arrives in a branch pipe 5, follows on a hollow mark 9 at a tube 12 of the wheels of Segner 10, 11 and through their nozzles 13 got into the volume of the cultural liquid, causing its aeration, on the one side, and gives rotating movement of wheels to the opposite sides - from another.

Wheels 10, 11 due to the reference carry out mechanical mixing of the cultural liquid which intensity, with other equal conditions, is proportional to their thickness, and the shovels 11 - extinguish the foam created by air and ability to live of bacteria.

As the wheels of Segner 10, 11 in axial section have the П-shaped form, instead of the rectilinear form in a prototype, and are characterized by height Н a thickness (height), and so the volume of the layer of the cultural liquid mixed by them increases several times in comparison with a prototype owing to what the intensity of mechanical mixing increases.

The intensive mass exchange accelerates growth of the microorganisms and leads to productivity growth.

Except increasing of the intensity of mechanical mixing, the aeration of the cultural liquid as the П-shaped top wheel of Segner of the offered forms carries out it on much wider range of depth which also assists to the productivity increase.