Nepreluk O.A. Kushnir K.G., Bobkova S.A.

Crimea state medical university named after S. I. Georgievsky, Simferopol.

State of cytochemical spectrum of peripheral blood neutrophiles of orthopedic patients with the syndrome of intolerance to acrylic plastics.

One of the most important problems in modern prosthodontics is the problem of intolerance of materials used in proshtetics: in particular plastics.

Studied the level of the enzyme succinate dehydrogenase and lactate dehydrogenase after prosthetics of the patients. Activity SDG decreased by 15,2 % and LDH- incresed by 13,5 %.

One of the most important problem is the problem of intolerance to the materials used in medicine.

Simultaneously with the increase of the level of people using dental prosthesis. Increased the level of patients who can not use them for various reasons.

Clinically, patients have marked changes in the tissues of the mouth , due to both allergic and non-specific inflammation, burning and other non-pleasent feelings. In the mechanism of allergic stomatitis on plastic materials denturesmain role belongs to action of sensitized lymphocytes. The general mechanism is as follows: in response to the allergen from entering the body are formed so-called sensitized lymphocytes. This lymphocytes is T-lymphocates;the cell membrane has the structure called antibodies, which connected with antigen. During the second attack of antigen the antibody connect with sensitized lymphocytes. That leads to morphological , biochemical and functional changes in lymphosytes. These changes apperance as blast transformation, proliferation secretion of different mediators, so-called lymphokines. Sensitized lymphocytes affect the direct cytotoxic and decrease the activity of the cells. This all revealed as inflammatory reactions of productive type. The main role in development of allergic reaction has the reactivity of the body.

The reactivity of the body determine the character of alergic disease. Its can explain that the allergy develope in special group of people. Determined that the group of risk compose the patients, having gastrointestinal diseases, medical and dietary allergy, bronchial asthma.

The main marker indicators are used to determine inflammatory, allergic and destructive changes are succinate dehydrogenase ad lactate dehydrogenase enzymes involved in the processes of glycolysis. The increase of these indexes in all cases is direct point into development of allergic and destructive processes.

Materials and methods

Comprehensive evalution and treatment of patients in need of orthopedic treatment with the use of removable plate dentures, conducted at the Department of Proshtetic Dentistry Medical Center CSMU. Were examined 45 patients(including women 23; men – 22), which were established partial dentures of material “Ftorax”.These patients were treatment group(aged 45-75 years).

The composition of the control group consisted of 40 patients between the ages of 25-65 years.

Comprehensive evalution of patients conducted by schema, collected medical history, examination of the oral cavity and periodontal examination of alveolar bone in the area of missing teeth carrying out the the clinical and labaratory methods of examination, formulation of diagnosis.

The learning the activity of dehydrogenase and enzymes of Krebs cycle and glycolisis.: succinate dehydrogenase ad lactate dehydrogenase as a non specific indicator of cells damage. Is known, the succinate dehydrogenase ad lactate dehydrogenase are main cell enzymes.

Results of investigation

Cytochemical analysis of the perfomance of peripheral blood neutrophils in patients and controls showed that the activity of succinate dehydrogenas(SDH) was 1.84±0,07 conv. u, the activity of lactate dehydrogenase (LDH)2,3±0,14 conv. u. After installing the removable plate prostheses ”Ftorax” group of patients who have developed intolerance syndrome akrilantam to aerobic oxidation (LDH activity)in the peripheral blood neutrophils was reduced by 15,2 %(P<0,05)and anaerobic glycolysis (LDH) increased by 13,5% (P<0.05).These figures indicate a significant allergic-destructive processes in the oral cavity.

Conclusions

1. Revealed, that in many cases after installation of removable implants, made from acrylic plastic developed syndrome of intolerance, that accompanied by inflammatory processes in peridontal tissues.

2. Analysis of cytochemical indexes of peripheric blood neutrophils showed, that after installation of removable implants made from materil “Ftorax” the activity of succinate dehydrogenase decreased (15,2%) and lactate dehydrogenase – increased by 13,5 %.