Karachun V.V., Trivailo M.S., Mel’niсk V. N.

National Technical University of Ukraine «KPI»

THE REVERSE-TRANSLATIONAL TRANSFERENCE

The utility model concerns to biotechnology and can be used for the cultivation of cells in liquid mediums at manufacturing of vaccines and biologically-active substances.

The known device for the cultivation (AC) of cells which contains the cylindrical case with technological branch pipes, a hollow shaft with regulated on frequency of the reference an occasion, the mixer fixed on a shaft in a shape of the four-bar articulate mechanism with shovels, the filter and the aerator (А.c. The USSR № 1633814, С12 M3/00, 1995).

The lacks of this device are caused by complexity of a design owing to presence in it of the regulated occasion and quite difficult form of a mixer, passive mixing of a working liquid on its height, pollution of a working liquid by products of a pulling down which result from a mechanical friction in the articulate mechanism and damage of cells.

There is also known design of the device which contains the cylindrical case with the technological branch pipes, a shaft with the external carving placed along an axis of the case, the mixer in the form of located under a right angle to an axis of the case of a round plate (disk) with the guide screwed on a shaft carving, an aerator, and also a reversible drive (for example, the patent of Ukraine for useful model № 12121, С12 M3/00, 2006).

This device is the closest to the utility model for a technical essence and achievable effect and accepted as the nearest analogue.

Known AC has more simple design from previous and provides active mixing of a working liquid in all its volume, but it does not discharge pollution of a working liquid by pulling down products as contains zones of a mechanical friction on surfaces of the mixer, and damage of cells which serves as the basic lack and reduces quality of a finished product.

The basis of useful model is a task of improvement of the device by withdrawal of a mechanical friction in volume of a working liquid. It allows to liquidate to a certain measure of pollution of a working liquid by pulling down products (at preservation of active mixing of a liquid and simplicity of a design), and also damage of cells which are given by possibility to raise quality of a finished product.

The given task is solving by that in AC which contains the cylindrical vertical case with the technological branch pipes, a shaft placed along a axis of the case, the mixer in the form of a plate attached to a shaft, an aerator and the reversible drive, according to the utility model the new is that the plate settles down along a axis of the case and is radially fixed on one of the shaft parties, and the case is equipped by a vertical partition which blocks a backlash between surfaces of the walls of the case and the shaft.

The specified distinctive signs, in comparison with the nearest analogue, discharge a mechanical friction in volume of a working liquid, and its consequences - pollution of a working liquid and damage of cells which are improved accordingly by quality of a finished product.

In the most conductive performance variant of the offered AC, at least nearby to a wall of the case the partition part can be executed punched, that takes up to occurrence of stagnant zones and intensifies mixing, and the plate and a partition are located thus which block technological level of a working liquid - owing to what is provided defoaming, and so and the acceleration of the cultivation process.

The essence of the utility model explains by drawings where it is schematically represented: on Fig. 1, a – longitudinal section of the AC, on Fig. 1, b – section А-А on Fig. 1, a.

AC contains the cylindrical case 1 with a branch pipe 2 for introduction of a nutritious liquid and a sowing material, a branch pipe 3 with an aerator 4, a branch pipe 5 for removal of the cultural liquid, and a branch pipe 6 for removal of the fulfilled gas. Along the axis of the case 1 the shaft 9 is located, attached to a motor-reducer 7 with command-reversible device 8, to which the mixer 10 is motionlessly attached. The mixer 10 is executed in the form of a plate 11 with "a" width and length «l» which is located along an axis of the case 1 and it is radially placed on one of the parties of a shaft 9 and makes with a wall of the case a backlash «b» for blocking of a working liquid. The case 1 is equipped by the vertical partition attached to its wall 12. The partition 2, except continuous, can be executed punched, at least in its pressed parts 13 and together with a plate 11 technological level blocks 14 working liquids 15 on size «Н» for maintenance of the defoaming.

The AC works in the following way.

In preliminary sterilized AC in the case 1 through a branch pipe 2 enter a nutritious liquid and a sowing material (the subculture) then in a separator 4 inject the gas for aeration of the cultural medium and include the command device which signal puts in action the motor-reducer 7 and a shaft 9, which confine with a corner «α» gives an reverse-forward movement to the plate 11. Moving in the circular direction, for example, clockwise 16(Fig. 1, b), the plate makes a pressure “P” on the working liquid 15, which is contained between it and the partition 12, due to what its flow through the gap “b” and the active mixing is occurring.

AC, unlike a prototype, in the volume of a working liquid of the offered DC there are no elements (details) with a mechanical friction its pollution by products of a pulling down and damage of cells by a friction is eliminated owing to what quality of a finished product increases.

The offered design is simple in realization and, except the improvement of a quality, reduces power inputs by mixing owing to elimination of a mechanical friction in volume of a working liquid.