Mel’niсk V. N., Trivailo M.S., Karachun V.V.

National Technical University of Ukraine «KPI»

THE SYSTEM OF STEMS FOR MASS-EXCHANGE

The utility model concerns to the biotechnology and can be used for the cultivation of cells in liquid mediums by manufacture of viral vaccines and biologically active substances.

The known device for the cultivation of cells (DC), which contains the cylindrical case with the bottom from non-magnetic material and the technological branch pipes, the established disk executed from a magnetic material at the bottom of case with the central basic core on its one part, and also a drive of a disk in the form of electromagnets located on a circle under a bottom of the case in regular intervals, which are consistently connected to the power supply (for example, А.с. The USSR №1726508, С12 M3/00, 1992).

The lack of this DC consists in low efficiency of mixing which leads to decline of productivity of the cultivation and product manufacturing.

The specified lack is caused by passive mixing of the cultural liquid in its top and average layers as the disk located on the bottom of the case has no elements directly co-operating with them.

The closest to the utility model for a technical essence and achievable effect and accepted for the nearest analogue DC which contains the cylindrical case with a bottom from non-magnetic material and the technological branch pipes, a disk executed of a magnetic material established on the bottom of the case with the central basic core on its one part and the mixing element in the form of a core on its opposite part, and also a drive of a disk in the form of system of electromagnets located on a circle under a bottom of the case which are consistently connected to the power supply in regular intervals (for example, the patent of Ukraine for useful model № 12120, С12M3/00, 2006).

The lack of known DC consists also in low efficiency of mixing, especially in the bottom layers of a liquid which inevitably leads to decline of productivity of the cultivation.

The specified lack is caused by that the mixing element in the form of the central core does not provide active mixing of a working liquid in its average and benthonic parts.

The basis of the utility model is the task of improvement of DC in which the intensification of the mass exchange of a working liquid in its average and benthonic parts which raises intensity and uniformity of mixing is provided by introduction in a design additional mixing elements and their eccentricly slanted arrangements and serves productivity growth.

The given task is solving by that in DC which contains the cylindrical case with a bottom from non-magnetic material and technological branch pipes both placed at the bottom of the case and the disk executed from a magnetic material with the central core on one part and central mixing core on the opposite side, and also a drive of a disk in the form of system of electromagnets located on a circle under the bottom of the case which are consistently connected to the power supply in regular intervals, according to the utility model the new there is that the disk is equipped by additional mixing cores which are located on a circle in regular intervals and are placed slanted to a disk axis.

The disk equipment by additional cores which are placed on a circle in regular intervals and are aslant focused to a disk axis, intensifies the mass exchange of a working liquid, especially in its bottom and average layers which raises intensity and uniformity of mixing owing to what growth of cells is accelerated, and also intensity of the cultivation increases.

The offered DC is represented on the drawing.

DC contains the cylindrical case 1 with a bottom 2 of non-magnetic material and a cover 3 and technological branch pipes 4 for leading and removal of a working liquid.

The mixing disk 5 is located on the bottom 2 of the case 1 and executed from a magnetic material with the central basic core 6 on one (bottom) part and the centrally fixed mixing core 7 on another (top) part. The core 7 exceeds level of a working liquid 8 in the case 1 in height. A disk 5 is equipped by additional mixing cores 9, which are located on a circle with the diameter "D" in regular intervals and placed aslant under a corner " α " to a disk axis.

The drive of a disk 5 is carried out from the electromagnets 10 established under the bottom 2 on a circle in regular intervals which in a pulse program mode are connected consistently to the power supply 11. Cores 7 and 9 in cross-section can have the round or flat form. The flat form of cores with their identical weight with round-one, increases the area of their interaction with a working liquid which in addition improves the mass exchange, under the identical other conditions.

DC works in the following way.

At inclusion of an occasion the electromagnets 9 generate a rotating magnetic field which draws a disk 5 owing to what it comes at oscillatory sinusoidal movement round the bottom point of a basic core 6. Fluctuating disk 5 and a core carry out moving of the cultural liquid 8, a disk 5 and a core 6 - mainly benthonic layer, a core 7 - top, and the aslant located cores 9 - average and bottom layers.

In comparison with the nearest analogue, the offered DC provides at the expense of the additional mixing of the cultural liquid with cores 9, the increasing of uniformity of distribution of nutrients and cells which creates additional conditions for growth of cells and serves for the growth of productivity of the process.