Pashkovska O.
Chernivtsi lyceum ¹
3, Ukraine
The
Prospects of Usage îf Palino-monitoring
of the Atmosphere Aerosol Pollution Caused by Micro- and Ultra-dispersed
Particles
The
problem of air pollution by various anthropogenic sources is considered to be one
the most essential ecological and social issues at present. According to the
NASA data, Ukraine belongs to the countries with most polluted environment. The
contaminated air annually kills nearly 500 Ukrainians per 1000 square
kilometers, i.e. 301 thousand people.
The
size of the atmosphere aerosol particles ranges from about several nana-meters
up to a few dozens micro-meters. Whereas the particles over one micro-meter
large consist mostly of mineral dust, sea salt and other particles of
biological origin, the ones having less than one micrometer in diameter are
supposed to be the result of different technological processes. They may
present a considerable threat on condition that they contain admixtures of
heavy metals and poly-cyclic aromatic hydrocarbons. The concentration of these
particles considerably ranges during a day, or even a week, particularly on the
territories with heavy traffic. This generates another serious problem, since
numerous substances, absolutely safe in their natural form, get extraordinarily
toxic and dangerous after they are ground to micro- and nana- size. The latter
danger can be explained by the fact that the particles assume certain
properties and biological affects that have not been characteristic for them
before. For instance, they may cause genotoxicity and damage DNA, thus
generating various nuclear aberrations ( V.F. Moskalenko, O.P. Javorskuj, 2009;
I.S. Chekman and coauthors, 2011).
Taking
into account the above mentioned phenomena, particular emphases is being laid on
the investigations, closely connected with the assessment of the state of the
urbanized environment. When supplemented with some information concerning
physical and chemical control, the bio-monitoring data come very close to the
adequate assessment of the ecological situation. In this case, we might use
recent pollen kernels of trees and herbs (palino-monitoring) as the principle
objects of bio-monitoring as they are most subject to the negative affect of
different constituents of the atmosphere pollution ( O.F. Dzyba, 2007; N.A.
Berezina, N.B. Afanasjev, 2009). Particularly, it has been ascertained that
plants produce a great number of teratomorphic and sterile pollen kernels when
they grow in ecologically polluted environment (Erokhina I.S., Elkina N.A.,
2011; V.P. Bessonova, 2013). The research of certain morphological
peculiarities of pollen kernels does not only prove the presence of different hameto-pathogenic
substances in the environment, but also provides a comparatively accurate
assessment of polluted zones without applying the method of direct mutation
accounting. Besides, there is no need to apply expensive equipment in
conducting research of this sort. The basic advantages of this method are the
speed with which the experiments are performed and the ability to screen as
many samples as possible.
The
objective of the investigation under discussion lay in working out the method
of palino-monitoring of the aerosol air pollution by micro- and ultra-dispersed
particles with the application of the parameters of nuclear chromatin coloring
in pollen kernels (on the example of
Chrysanthemum Morifolium).
A
complex assessment of the dispersed constitution of the aerosol air pollution
on the territory of Chernivtsi has been performed. The monitoring of the minute
air particles has been conducted by means of the portable device Met One
GT-321. The particles with the size of 0.3, 0.5, 1.0, 2.0 and 5.0 micro-meters
have been registered during the research. Depending on the obtained results we
have differentiated zones with high and moderate pollution, as well as the
conventionally clean territories.
The
above mentioned territories have provided our research with the required plants
(Chrysanthemum Morifolium). The pollen kernels have been separated from the
polls; wiring in the ascending ethanol battery and paraffin pouring at the
temperature 560C (in order to preserve the nuclear parameters) have
been performed in the course of the investigation. The obtained paraffin
microtomic cuts 5 micro-meters thick were painted in azure-2-eosin which
enables to achieve the contrast coloring of the nuclear chromatin. The substances
have been examined in the traversed light by the means of the microscope Delta
Optical Evolution 100 with the application of the glass lens 40-x. Then the
digital copies have been made with the help of the digital camera Olympus SP
550 UZ.
The
arithmetic mean of the coloring optical density (in optical density relative
units in the range “0 – 1”), as well as the index of the mean-square deviation
of the coloring optical density have
been calculated by means of the computer program GIMP (the GNU Image
Manipulation Program) Portable 2.213. The calculation of the coloring optical
density variations coefficient (in percent) has been made by means of dividing
the magnitude of the mean-square coloring optical density deviation by the
arithmetic mean of the coloring optical density multiplied by 100.
The
coloring optical density variation coefficient of the pollen kernels nuclear chromatin
of the Chrysanthemum on the investigated territories made 11.6±0.53%.
The
same index in the zone of moderate pollution must have exceeded the basic one
by 47.8% (P<0.001). It should be noted that the coloring optical density
variation coefficient of the nuclear chromatin of the plants’ pollen kernels,
found in the area of aerosol pollution, twice exceeded the one from the
conventionally clean zone (with a high verity degree P<0.001).
The
growth of the coloring optical density variation coefficient proves the
increase of the pollen kernels nuclear heterogeneity as the result of the euchromatin
reduction. Besides, we may draw a conclusion that the increase of the pollen
kernels nuclear heterogeneity may reflect the active processes of apoptosis,
which are traditionally accompanied by the phenomena of the nuclear chromatin
condensation and margination.
Thus,
the suggested method of palino-monitoring with the application of the
parameters of the pollen kernels nuclear chromatin coloring gives the
opportunity not only to perform the indication of the air aerosol pollution in
a certain area, but also to determine the degree of the nuclear activity
regarding the DNA involvement in various synthetic processes.