Bobkova S.A., Makarenko V.N., Kazinina E.N.

Crimea state medical university named after S. I. Georgievsky, Simferopol.

Data of indexes of periodontal destruction in orthopedic patients having bridges based on implants

In the study of periodontal standard methods (survey, examination instrumental studies, radiography) often used an index evaluation system, which allows to give a comprehensive assessment of periodontal disease. Indexes are normally not require sophisticated diagnostics, it based on an assessment of easily identifiable changes mouth.

Cytomorphometry is cytology section, which is based on a quantitative, morphometric evaluation of cell populations in cytograms. This method allows the correlation relationship between required cytomorphometric indicators - index destruction and inflammatory and destructive index, the character and severity of the pathological process in the developing tissues studied.

Index sample are widely used in dental practice, because the measurement results help the doctor determine the strategy and tactics of further action.

Thus, the aim of our study was to monitor the cellular elements cytomorphometric periodontal after installing bridges relying on implants inorthopedic patients.

Methods of investigation

Were examined 26 people of both sexes, requiring orthopedic treatment using bridges.

Collection of material for the study was carried out on 1, 3,6,12 months after prosthesis.

Exploring the cytograms by gums and mathematical processing of the data was performed by the method of A. Grigoryan.

Application cytomorphometric method indicate for early inflammatory complications in the mucosa of the gums, determining the character and severity of the inflammatory process and evaluate the effectiveness of hygiene and preventive measures.

Cytomorphometric method of research was carried out on prints gum taken from the lingual or buccal surface in the area adjoining it to the implant neck (zone interface), employe support for the prosthesis. Prints were collected from the gingival sulcus, after 4 (± 1) hours after brushing.

For prints used target - sphenoid fragment gum - eraser to the size of its narrowest part of no more than I mm. Targets were stored in Petri dish, filled with 50% ethanol solution. Before taking the gum target prints were dried with an air jet from the air gun is then readily introduced into the gingival sulcus tightly pressed to the inner cheek □ implant surface, and a symmetric outer portion gums.

Prints were applied to skim slide. Dried and stained by the method of Romanovsky-Giemsa.

Then the preparation was studied under a microscope at high magnification, ocular X10 (X20) and X40 .

Field selection is arbitrary,but there should be at least 3. Further,for calculation of the index determines the amount of epithelial cells(regardless of their degree of maturity) and the number of neutrophils in the field of view.

The index has the following range of values:12,09-15,03 - norm; 15,04-22,05 - weak inflammation of expression, 22,06 and more - moderately and strongly marked inflammation. Noted that gingival and prints normally present fagir cells, but the ratio in inflammatory processes of epithelial cells and connective tissue changes sharply upward connective.

During the analyzing the performance index cytomorphological periodontal destruction in the control group was 12,53 ± 0,26 conv.

By 1 month after the installation of bridges implant there is a slight and not statistically significant bearing the character (P> 0.05) increase in the index periodontal destruction by 8.8%, which was 13,64 ± 0,52 conv

By 3 months after the current orthopedic treatment index periodontal destruction was 13,12 ± 0,41 conv , which was 4.7% ( P> 0.05 ) higher than the control .

In long-term follow 6-12 months, the index of periodontal destruction as close to the benchmarks and was 12,86 ± 0,33 conv.

Conclusions

The carring out the analysis cytobiochemical study allowed to conclude that the installation of bridges (made of acrylates ) implant has no significant changes in the periodontal tan patients , as evidenced by the performance index periodontal destruction .