Mel’niсk V. N., Trivailo M.S., Karachun V.V.

National Technical University of Ukraine «KPI»

THE GROWTH OF PRODUCTIVITY AT PRODUCING BIOLOGICALLY-ACTIVE SUBSTANCES

The model refers to microbiology and may be used for cultivation of microorganisms in liquid environments at producing biologically-active substances and vaccines.

It is known the plant for cultivation of microorganisms (PCM) which contains a frame with technological branch pipes, in which cavity there are situated cameras for bringing of nutrient substance and cultivation of biomass, and also situated on the shaft bleding mixer with enveloping pipe (for example, patent of Russia № 2012593, С12 М1/04, 1994).

The fault of this PCM consists in complication of its structure.

It is known the PCM which contains two cameras with technological branch pipes and devices for supply of sterile air, connected among themselves in the bottom by flexible conduit, and also a mechanism of reverse-translational movement of cameras in vertical plate (for example, A.c. of USSR № 1131899, С12 М1/00, 1984).

This PCM is the nearest to the utility model about technical essence and reaching effect and is taken as prototype.

The fault of the known PCM consists in reduction of productivity in consequence of absence of mixing of working liquid with air, that decreases the presence of air in it, and that is why it slows down the growth of microorganisms.

In the basis of the utility model it is putted the problem of improvement of PCM, in which by equipping the ends of conduit by additional elements, it is providing mixing of part of working liquid with air, that leads to growth of productivity.

The putted problem is solved by that in the PCM which contains connected among themselves in the bottom by flexible conduit filled with working liquid (liquid or gas) two cameras with devices for supply of sterile air and mechanism of reverse translational movement of cameras in vertical plate, according to the utility model the ends of the conduit are equipped with added to them pipes, in which wallsides on the joining ends the through holes are made.

The mentioned difference ensures the overflowing of part of working liquid in the volume of air, that is absent in prototype and leads to growth of productivity.

The declared PCM is schematically presented on fig. 1.

The PCM contains two cameras 1, 2, which are connected in bottom among themselves by flexible conduit 3 with the device 4 for overflowing and filling up of working substance. Cameras 1, 2 are equipped with situated in their top devices 5 for supplying of sterile air and joined to the drive of their reverse translational movement in vertical plate. The drive contains reversible motor-reducer 6 with directed block 7 and drum 8 which envelopes turned over the blocks 9 and joined to cameras 1, 2 rope 10.

The ends of the conduit 3 are equipped by joined to them pipes 11, 12 which have holes 13, 14 on joining ends. The ends of the pipes 11, 12 are situated in bottom and top of cameras, and their holes 13, 14 ensure, as in prototype, free overflowing of working liquid between cameras. Pipes 11, 12 may be used as the whole with conduit 3 (not shown).

PCM works in next way.

The previously sterilized and filled approximately a half of their volume by nutrient liquid with inoculator (working liquid) cameras 1, 2 by engaging the motor-reducer 6 come into reverse-translational movement and realizes the cultivation of placed in it cultures of microorganisms. When liquid transfers it overflows through the conduit 3 from one camera to another, for example, from camera 1 into camera 2. Flowing out the hole 13 from camera1, working liquid with help of pipe 12 interflow into camera 2. Meanwhile part of the liquid flow out through the hole 14 into bottom part of camera 2, and another part of liquid in consequence of inertia continues to move through the pipe 12 and flows out in the top part of camera into the volume 15 of air in the form of streams 16 where its parts, for example, one of them is 17, in addition saturates with air and mix among themselves. The overflowing of the liquid in reverse way goes analogous.

In the declared PCM, different to the prototype, the overflowing of working liquid into cameras realizes by two streams, one of which is situated it the volume of air, that is why the saturation of liquid by air and its mixing increase, that stimulate the growth of microorganisms and lead to rise of productivity.