Mel’niсk V. N., Trivailo M.S., Karachun V.V.

National Technical University of Ukraine «KPI»

THE circulation of culture broth

The utility model refers to microbiology and may be used for cultivation of microorganisms in liquid environments at producing biologically-active substances and vaccines.

It is known the plant for cultivation of microorganisms (PCM) which contains a frame and turning in inclined plate platform with vessels for cultivation, and also a drive (for example, A.c. of USSR № 1731801, С12 М1/10, 1992).

The fault of this PCM consists in complicacy of use, so far as it requires for packing of working liquid at using.

It is also known the PCM which contains two cameras with technological branch pipes connected among themselves in the bottom by flexible conduit, with branch pipes for joining the flexible conduit and devices for supplying of sterile air, and also a mechanism of reverse-translational movement of cameras in vertical plate (for example, A.c. of USSR № 1131899, С12 М1/00, 1984).

This PCM is the nearest to the utility model about technical essence and reaching effect and is taken as the nearest analogue.

The fault of the known PCM consists in low effectiveness of mass exchange of working liquid that is caused by placing of branch pipes for joining of flexible conduit parallel to cylindrical walls of cameras and decreases the productivity.

In the basis of the utility model it is putted the problem of improvement of PCM in which by means of change of placing of branch pipes for joining of flexible conduit it ensures the extra transference, and thus the mixing of working liquid, that causes the growth of productivity.

The putted problem is solved by that in the PCM which contains two cylindrical cameras with technological branch pipes connected among themselves in the bottom by flexible conduit, with branch pipes for joining the flexible conduit and devices for supplying of sterile air, and also a mechanism of reverse-translational movement of cameras in vertical plate, according to utility model the new is that branch pipes for joining of flexible conduit are situated in cylindrical walls and are placed touching to them (to walls).

The placing of joining branch pipes in cylindrical walls and touching to them disposition, in contrast to parallel disposition in the nearest analogue, ensures at transfusion of working liquid its circular movement in cameras, and that is why, extra mixing, in consequence of what the evenness of distribution of working substances and microorganisms in working liquid increase, and it leads to increasing of productivity.

On fig. 1, а it is schematically presented the proposed PCM, general view; on the fig. 1, b – the crosscut А-А on the fig. 1, а.

The PCM contains two cylindrical cameras 2, 3, which are connected among themselves by flexible conduit 1 with devices 4, 5 for supplying of sterile air 6. Cameras 2, 3 are joined to the mechanism of their discontinuous reverse translational movement in vertical plate, whose drive contains reversible motor-reducer 7 with control block 8 and drum 9, which envelopes putted over the blocks 10 and joined by its ends to cameras rope 11.

Cameras 2, 3 have technological branch pipes 12 for withdrawal of working liquid and branch pipes 13 for joining the flexible conduit 1. Branch pipes 13 are situated in cylindrical walls of cameras 2, 3 and are located touching to them (fig.1, b). The flexible conduit 1 has a cock 14 for pouring off the contents of cameras and technological overlap of conduit.

The PCM works in next way.

One of previously sterilized cameras, for example at the shown on the drawing (fig. 1, а) posture camera 2 is filling up with nutrient liquid and sawing material (working liquid), whereupon the cock 14 on the conduit 1 is opening and the cultivation of the placed in nutrient liquid cultured is realized. The opening of the cock 14 leads to overflowing of working liquid by conduit 1 from camera 2 into camera 3, increasing its level of “H” in camera and forcing out of it air 6. At this time, the flowing out from branch pipe 13 stream 15, falling on the curvilinear (cylindrical) wall of camera, twists the working liquid which is located in it in circular straight, provoking its extra mixing.

After filling up the camera 3 with working liquid to the necessary level, control block 8 turns up the motor-reducer 7 and changes the position of cameras in vertical plate: camera 2 – in bottom position, camera 3 – in top position, in which they stay to the determined for overflowing time. The overflowing of working liquid between cameras in changed position occurs in reverse straight, from camera 3 into camera 2, by earlier described way.

After necessary number of cycles of transference and time of overflowing the contents of cameras are pouring off through the cock 14 for further use.

In consequence of that at overflowing of working liquid between cameras 2, 3 it happens its extra mixing in circular straight of cameras, that is absent in the nearest analogue, the intensity of mass-exchange rises, that increases the evenness of distribution of nutrient substances, and it stimulates the growth of microorganisms and leads to growth of increase of productivity

The proposed PCM is ease to realize and do not require for substantial expenditure on producing.