Медицина / 6. Экспериментальная и клиническая фармакология

Galkin O.Yu., Dugan O.M.

National Technical University of Ukraine “Kyiv Polytechnic Institute”

Peremogy Avenue 37, Kyiv, 03057, Ukraine

Biological activity of phytopreparation THAT is designed for alopecia treatment

At earlier stages of research we have conducted pharmacological and phytochemical design of herbal preparation for the treatment and prevention of alopecia. Phytopreparation is complex tincture of collection of medicinal plant material, consisting Arctii Radices, Sophora Japonica fruits, Acorus Clamaus rhizome, Folia Urticae Dioicae and Humulus Lupulus. Biologically active substances of medicinal plants have analgetic, antiitch, epithelium creating, and antiseptic properties. They restore the structure and functions of the skin, improve blood circulation in the capillary system of the skin, it stimulates metabolism and trophic processes, improve supply scalp.

It should be noted that the main criteria for successful implementation of medicines in clinical practice is its safety and its specific activity. Thus, the study has been determined to the study of biological and pharmacological activity of herbal preparation.

Study antiextravasate activity of phytopreparation have been performed on the “rigid” model of inflammation, which is swelling of the limbs caused subplantaric injection of 2% formalin solution. Rating of antiextravasate activity also have been obtained on the model of adrenalin pulmonary edema. Study of phytopreparation influence on the proliferate component of inflamed reaction have been evaluated on the model of “cotton granuloma”. The analysis revealed anti-inflammatory activity of phytopreparation by antiextravasate and antiroliferate effects, due to the presence of flavonoids and tannins.

It has been found that mouse tissue macrophages derived from peritoneal exudate, become activated when they are cultivated in vitro in the presence of phytopreparation.

Activation of macrophages showed by changes in their size and shape, as well as their metabolic and enzymatic activities. It has been established that phytopreparation increases the ability of macrophages production of oxidation metabolites at a meeting with the components of microorganisms. Incubation of peritoneal macrophages in the presence of phytopreparation for 24 h has not affected the level of spontaneous chemiluminescence, but has increased markedly (26%) the ability of cells to oxidative metabolites production in response to zymozan (fig. 1). This increase in chemiluminescence activity occurs gradually in the range of concentrations of phytopreparation from 50 to 200 mg/ml and maximal effect has achieved at concentrations of 300 mg/ml.

Fig. 1. The intensity of chemiluminescence of peritoneal exudate cells in response to zymozan after preliminary cultivation of cells in the presence phytopreparation

These data agrees well with the concentration of phytopreparation that leads to maximal activation of macrophages, judging from their morphological characteristics. In the study of secretion of monocytic cytokines during their cultivation in phytopreparation containing medium has been installed pronounced effect on production of IFN-γ and IL-2 (fig. 2). There has no marked effect on the secretion of interferon IFN-α, tumor necrosis factor-α, IL-1 and IL-8.

Fig. 2. The contents of cytokines in culture medium of peripheral blood cells after 12 hours (IL-2) and 48 hours (IFN-γ) of incubation with medications

Conclusions.

1. The investigations of acute toxicity of phytopreparation identified its LD₅₀ intraperitoneal (142.0 ml/kg). In examining the impact phytopreparation the skin and mucous membranes was not detected abnormalities. Phytomedication was assigned to the small hazardous class substances. It has been found anti-inflammatory activity by phytopreparation antiexudative and antiproliferative effects, due to the presence of flavonoids and tannins.

2. It has been found moderate immunostimulating activity of phytopreparation (in conditions in vitro).