Medicine/8.Morphology
Ulyanov V.O.
Age features of the
thiol-disulfide system of the myometrium in posterity of the irradiated rats
Pathology of contractive
function of uterus is one of main problems of obstetrics. It is the principal
reason of maternal and perinatal morbidity and death
rate. The mechanisms of pathology of contractive function of uterus caused by the
small doses of radiation are not enough studied. It was founded that the
components of the thiol-disulfide system take part in providing contractive
activity of uterus. There are some works devoted the changes of the thiol-disulfide
system of myometrium at the action of small doses of radiation. Works are
devoted the changes of the thiol-disulfide system in ontogenesis of descendants
of the radiation-exposed animals not discovered by us. This subject has not
been fully researched before.
Purpose. To research
the state of the thiol-disulfide system of the myometrium on the different
stages of ontogenesis of animals, got from the radiation-exposed predecessors.
The contractive
activity of uterus was researched in adult experimental rats of the laboratory line of Vistar. Mature males
and females of rats were irradiated in total
dose 1,0 Gy. After completion of the irradiations the
rats were coupled and got posterity of first generation. State of the thiol-disulfide
system of the myometrium was probed in females, got from the radiation-exposed
rats, in 14, 30, 45, 60 and 90th time of their life.
Sulfhydril and
disulfide groups were discovered by the Barrnett and Seligman's method.
For the quantitative estimation of contents of sulfhydril and disulfide groups,
by the light microscope of "Carl Zeiss Axiostar plus" and digital video camera got the electronic
image of the probed micro slides. The integral absorbancy
of the painted components was determined with help of software „VideoTesT”.
It was established, that the thiol-disulfide
correlation in intact females on the different stages of the sexual development
was without changes. The contents of sulfhydril and disulfide groups in the
myometrium on the different terms of supervision changed.
In the myometrium of 14-daily rats at the
moment of completion of postnatal period, was high contents of sulfhydril and
disulfide groups. In 30 time of life the contents of sulfhydril groups
diminished comparatively with the previous term of supervision on 10,5 %. It is
the sign of their intensive using at the beginning of the sexual development of
the laboratory rats. But stability in the thiol-disulfide system was not
violated. The statistically reliable changes of correlation between sulfhydril
groups and disulfide groups were absence.
The contents of sulfhydril groups in the myometrium of 45-daily females
on the peak of the sexual development increased comparatively with the previous
term of supervision on 10,2 %. Thus contents of disulfide groups and their
correlation to sulfhydril did not change.
The contents of sulfhydril groups diminished
on 27,6%, disulfide - on 26,4% after achieving puberty. Their correlation was
saved at the level of 45-daily rats. The variations of contents of sulfhydril
groups on the different stages of the sexual development represent their active
role in the sexual development and changing of myometrium.
For females, got from
the radiation-exposed rats, were typical quantitative differences of contents
of thiol groups in the myometrium and some other their age-old dynamics. In a
period from 14th for 30th time of life found out diminishing of contents of
sulfhydril groups on 31,1%. From 30th for 45th their contents grew on 20,4% and
finally to the 90th days fell on 35,4%. Such changes are the sign of
instability of the thiol-disulfide system of the myometrium in descendants of
the radiation-exposed rats. It was established the decreasing of contents of
disulfide groups specifies on 30th time of life on 19,2%, comparatively with
14th day of the supervision. Diminishing of contents of disulfide groups and
sulfhydril is the sign of denaturation changes of proteins
molecules. The thiol-disulfide correlation of the myometrium during of all
stages of ontogenesis in descendants of the radiation-exposed rats was
decreased comparatively with intact rats.
Thus all of the stages
of the sexual development in descendants of the radiation-exposed rats were on the
background of dysbalance in the thiol-disulfide
system. It can damage the myometrium structure and its functional properties. Changes
in the thiol-disulfide system can cause the disorders of contractive activity
of uterus.
Conclusion.
The irradiation of males and
females of rats before a couple causes changes in the thiol-disulfide system of
their descendants of first generation on all stages of the sexual development.