Kolbasina M.V. , Kolbasina L. P., Fazel Hamid

Crimea State Medical University named after S. I. Georgievsky

“Monitoring of the state of cellular and humoral immunity for children with disadaption on the sanatorium-resort stage of rehabilitation ”

SUMMARY

This article is devoted to the immune monitoring of the children who often suffer from the long-term diseases along with the disadaptional syndrome while applying the complex methods of correction at the period the resort treatment.

Homeostasis and adaptation are interdependent processes that complement each other. So urgent is the study of adaptive reactions, which develop in patients receiving sanatorium-and-resort treatment reduction of the duration of these responses for the achievement of therapy in conditions of the resort are very promising. General adaptation syndrome facilitates the work of abused structures biosystems and therefore is reasonable and appropriate bio-energy.Distinguish the species and individual adaptation. Individual adaptation develops throughout the life, the body becomes vacant earlier resistance to a particular factor of the environment. The mechanism of individual adaptation consists in the fact that, in response to the action of a factor is violated homeostasis, and this activates the reaction, responsible for the adaptation to this factor - stress-reaction. Hyperfunction of the system, dominanting in this situation, through the genetic apparatus, forms the structural footprint, providing long-term adaptation.The reaction of the organism to environmental changes qualitatively different and can vary from physiological to the pathological. An important task in the optimization of the adaptation process is the development and application of methods and ways of increasing the non-specific and specific resistance of the organism, its adaptive capacity. With this purpose are used as therapeutic physical factors, as well as different medications - adaptogens, correcting the course of the adaptation process. The aim of our study was to investigate the immune status of the children with the syndrome disadaption, as well as monitoring of the state of cellular and humoral immunity when using complex methods of correction.

Material and methods

In this paper used the materials of examination of 53 children of both sexes aged from 9 to 13 years, located in the sanatorium-and-Spa rehabilitation in sanatoriums of the "Jubileinyi" and "Smarahdovyi "and having a history of diagnosis DCHB (long-term, and frequently ill), mostly chronic diseases nazofaringialnoy area. This, in particular, allergic rhinosinusopathy (28,6%), chronic tonsillitis(26,5%) adenoiditis(14,3%), chronic nasopharyngitis (10,2%), pollinoz(10,2%), vazomotornyi rhinitis(of 9.5%). The entire contingent of the surveyed was divided into 2 groups. The first group consisted of children receiving the traditional therapy (20 people - 37,7%), and the second group - 33 child (62,3%), receiving complex therapy according to our method (patent of Ukraine # 15779) and which includes the use of aromatherapy product "Polyol", the impact on the reflex zones by the needle applicator Lyapko’s and the appointment of immunomodulator " Amizon " in the dose of 0.125 mg 2 times a day for 2 weeks complex immunological studies of cellular immunity was formed from certain height absolute number subpopulations of lymphocytes, bearing the following clusters in surface differentiation markers (classification CD-claster the differentiation ): CD3 (a marker of the absolute majority of mature T - lymphocytes ), CD4 ( helper- inductor subpopulation of T lymphocytes ), CD8 ( killer - suppressor subset of T lymphocytes ), CD16 ( natural killer cells - NK), CD22 ( B- lymphocytes). To identify specific markers differentiating the method statement indirect reaction surface immunoflyuoristsentsii with monoclonal antibodies.State of humoral immunity was assessed by the concentration of serum immunoglobulin classes A (IgA) , and E (IgE). For this purpose a test system , designed for the quantitative determination of these antibodies in serum by enzyme-linked immunosorbent assay ( manufacturer , JSC \ \ \ " Vector-Best \ \ \" , Novosibirsk) with monoclonal antibodies with high specificity to these immunoglobulins. All obtained in the research process is handled by the digital material variation statistics deducing the Student test. Considered reliable indicators in the (P<0,05).

Results and Discussion

We evaluated the state of both cellular and humoral immunity in children, long-term and often ill received for the period of rehabilitation in sanatoria Institution Crimea. A similar trend was observed on the part of humoral immunity, where the concentration of IgA and IgE declined, and the indices of IgE in relation to the indicators on the moment of receipt of the sanatorium (P2) acquired a statistically significant in nature. In the subsequent periods of observation (10-15-e day) in this group of patients indicators of immune status closer to the test, getting the information at the beginning of the correction of disadaption statistically significant in nature (P2<0, 01). In determining the immune status of children belonging to the category of DCHB, arrived at the sanatorium-and-Spa phase of rehabilitation, found a violation of both cellular and humoral link of immunity, which we regarded as expressed by the stress reaction to the process of development disadaption. The use of the complex etiopathogenic -based therapies, including physical therapy and medication correction techniques, allowed to normalize the immune status of the children to the 5th day, the us was seen as the development of the adaptation process. We formulated and implemented in a number of resort establishments of the Crimea integrated methods of correction disadaption in children, long-term, and frequently ill, can be widely recommended for sanatorium-and-resort stages of rehabilitation.